The induction of ferrous sulfate or sodium acetate on mRNA expression of carotenoid genes in Haematococcus pluvialis

文献类型： 外文期刊

第一作者： Gao Zhengquan

作者： Gao Zhengquan;Meng Chunxiao;Gao Hongzheng;Su Yuanfeng;Zhang Xiaowen;Ye Naihao

作者机构：

关键词： Haematococcus pluvialis;astaxanthin;ferrous sulphate (FeSO4);sodium acetate (sa);carotenoid genes qRT-PCR

期刊名称：RESEARCH JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.233； 五年影响因子：0.252 ）

ISSN： 0973-6263

年卷期： 2013 年 8 卷 6 期

页码：

收录情况： SCI

摘要： Haematococcus pluvialis accumulates large amounts of astaxanthin under a variety of environmental stresses. In this study, transcriptional pattern of eight carotenoid genes involved in astaxanthin biosynthesis exposed to ferrous sulfate (1 and 2mM named Fe1 and Fe2) or sodium acetate (20 and 40mM named sa20 and sa40) was separately revealed using qRT-PCR. Results showed that all eight carotenoid genes were up-regulated by ferrous sulfate or sodium acetate with different expression profiles. Ferrous sulfate induction had a greater effect on the transcriptional expression of ipi-1, pds, crtR-b and bkt in Fe1 and pds and bkt in Fe2 than on other genes and sodium acetate treatments had more influence on the transcriptional expression of ipi-1, ipi-2, psy, lyc and bkt in sa20 and ipi-1 and psy in sa40 than on other genes. Furthermore, astaxanthin biosynthesis in the presence of ferrous sulfate might be up-regulated by ipi-1, ipi-2, pds, lyc, bkt and crtR-b at transcriptional level, psy at post-transcriptional level and by crtO at both transcriptional and post-transcriptional levels. Astaxanthin biosynthesis exposed to sodium acetate was upregulated mainly by ipi-1, ipi-2, psy, pds, crtR-b, bkt and crtO at post-transcriptional level and lyc at both transcriptional and post-transcriptional levels.

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