Pathogenicity and innate immune responses induced by fowl adenovirus serotype 8b in specific pathogen-free chicken

文献类型： 外文期刊

第一作者： Zhang, Xiaona

作者： Zhang, Xiaona;Liu, Liangliang;Fan, Jiahui;Xie, Jingjing;Jiao, Yaru;Ma, Deying;Zhang, Xiaona;Liu, Liangliang;Wang, Fangfang;Li, Huixin;Fan, Jiahui;Xie, Jingjing;Jiao, Yaru;Han, Zongxi

作者机构：

关键词： fowl adenovirus serotype 8b; speci fic pathogen-free chicken; phathogenicity; immune response

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2023 年 102 卷 8 期

页码：

收录情况： SCI

摘要： Fowl adenovirus serotype 8b (FAdV-8b), as causative agent of inclusion body hepatitis (IBH), poses a great threat to the poultry industry. Considering the importance of innate immune response in host against viral infections, we investigated pathoge-nicity of a FAdV-8b strain HLJ/151129 in 1-mo-old specific pathogen-free (SPF) chickens and immune responses of host to FAdV-8b infection in this study. The results demonstrated that no obvious clinical signs were observed in infected birds. Neither mobility nor mortality was observed in both FAdV-8b infected and control chickens, as well. However, hepatic necrosis and a small amount of inflammatory cell infiltration were observed by pathological analysis. Viral load was detected in bursa of Fabricius, cecal tonsils, liver, heart, spleen, Harderian glands, and thymus. Virus shedding and viremia generated as early as 3 days postinfection (dpi) (9/10) and reached the peak at 7 dpi (10/10). In addition, the infected birds had developed FAdV-specific antibodies at 7 dpi, and the antibody titers reached the peak at 14 dpi. Furthermore, the results demonstrated that the mRNA expression levels of most of toll-like receptors (TLRs), most of avian b-defensins (AvBDs), and cytokines [interleukin (IL)-2, IL-6, and interferon (IFN)-g], were significantly upregulated in most tissues at early phases of FAdV-8b infection, especially in liver and spleen. In contrast, FAdV-8b infection results in downregulation of TLR4, TLR5, and TLR21 expressions in some tissues of infected chickens. In addition, FAdV-8b infection upregulated myeloid differentiation factor 88 (MyD88), nuclear factor-kappa B (NF-kB) p65, and TIR-domain-con-taining adapter inducing interferon-b (TRIF) expres-sion in some tissues, while decreased NF-kB p65 and TRIF in spleen at both 72 hpi and 21 dpi. Taken together, these results confirmed that FAdV-8b could replicate in all investigated tissues of infected birds, and then, result in production of FAdV-specific anti-body titers. Meanwhile, the FAdV-8b infection indu-ces strong innate immune responses at early stage in chickens, which may associate with the viral pathogenesis.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序