Bioinformatics and Functional Analysis of OsASMT1 Gene in Response to Abiotic Stress

文献类型： 外文期刊

第一作者： Li, Mingyu

作者： Li, Mingyu;Wu, Longying;Shi, Yang;Afzal, Farhan;Jia, Yanru;Huang, Jin;Wu, Lijuan;Chen, Ji;Huang, Yanyan;Hu, Binhua

作者机构：

关键词： Abiotic stress; Rice gene; Bioinformatics analysis; Function

期刊名称：BIOCHEMICAL GENETICS （ 影响因子：2.4； 五年影响因子：2.2 ）

ISSN： 0006-2928

年卷期： 2024 年

页码：

收录情况： SCI

摘要： The study aimed to elucidate the functional characteristics of OsASMT1 gene under copper (Cu) or sodium chloride (NaCl) stress. Bioinformatics scrutiny unveiled that OsASMT1 is situated on chromosome 9. Its protein architecture, comprising dimerization and methyltransferase domains, showed significant similarities to OsASMT2 and OsASMT3. High expression in roots and panicles, along with abiotic stress putative cis-regulatory elements in the promoter, indicated potential stress responsiveness. Real-time quantitative PCR confirmed OsASMT1 induction under Cu and NaCl stress in rice. Surprisingly, yeast expressing OsASMT1 did not exhibit enhanced resistance to abiotic stresses. The results of subcellular localization analysis indicated that OsASMT1 plays a role in the cytoplasm. While OsASMT1 responded to Cu and NaCl stress in rice, its heterologous expression in yeast failed to confer abiotic stress resistance, highlighting the need for further investigation of its functional implications.

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