Epigallocatechin gallate improves the quality of diabetic oocytes

文献类型： 外文期刊

第一作者： Chao, Shuo

作者： Chao, Shuo;Li, Li -Jun;Lu, Jun;Zhao, Shu-Xian;Zhao, Ming -Hui;Huang, Gui-An;Yin, Shen;Shen, Wei;Ge, Zhao-Jia;Zhao, Lei;Sun, Qing-Yuan;Sun, Qing-Yuan;Zhao, Yong

作者机构：

关键词： Diabetes; Epigallocatechin gallate; Meiosis; Oocyte; Spindle

期刊名称：BIOMEDICINE & PHARMACOTHERAPY （ 影响因子：7.5； 五年影响因子：6.8 ）

ISSN： 0753-3322

年卷期： 2023 年 159 卷

页码：

收录情况： SCI

摘要： Background: Maternal diabetes compromises the quality and developmental potential of oocytes. Therefore, it is important to study how to ameliorate the adverse effects of diabetes on oocyte quality. Epigallocatechin gallate (EGCG) has a variety of physiological activities, including anti-inflammatory, antioxidant, and anti-diabetes. In the present study, we evaluated the effect of EGCG on the maturation of diabetic oocytes in vitro.Objective: Investigating the role of EGCG in restoring the adverse effects of diabetes on oocyte quality. Methods: Diabetes mouse model was established by a single injection of streptozotocin (STZ). Oocytes were collected and matured in vitro with/without EGCG in M16 medium.Results: Compared with control, diabetic oocytes have a higher frequency of spindle defects and chromosome misalignment, but EGCG effectively reduces the incidence of oocytes with abnormal spindle assembly and chromosome mismatches. Moreover, the abnormal mitochondrial membrane potential (MMP) of diabetic oocytes is significantly alleviated by EGCG, and the reduced expression of genes regulating mitochondrial fusion (Mfn1 and Mfn2) and fission (Drp1) in diabetic oocytes is significantly increased while EGCG is added. EGCG also decreases the higher level of reactive oxygen species (ROS) in diabetic oocytes that may be regulated by the increased expression of superoxide dismutase 1 (Sod1) and superoxide dismutase 2 (Sod2). EGCG can also reduce the DNA damage of diabetic oocytes.Conclusions: Our results suggest that EGCG, at least partially, improve the quality of diabetic oocytes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序