Increasing nitrogen absorption and assimilation ability under mixed NO3-and NH4+supply is a driver to promote growth of maize seedlings

文献类型： 外文期刊

第一作者： Wang, Peng

作者： Wang, Peng;Wang, Cheng-dong;Wang, Xiao-lin;Wu, Yuan-hua;Zhang, Yan;Sun, Yan-guo;Shi, Yi;Wang, Peng;Mi, Guo-hua

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关键词： maize; NO3-; NH4+ratio; N absorption; N assimilation; plant growth

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.8； 五年影响因子：4.8 ）

ISSN： 2095-3119

年卷期： 2023 年 22 卷 6 期

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收录情况： SCI

摘要： Compared with sole nitrate (NO3-) or sole ammonium (NH4+) supply, mixed nitrogen (N) supply may promote growth of maize seedlings. Previous study suggested that mixed N supply not only increased photosynthesis rate, but also enhanced leaf growth by increasing auxin synthesis to build a large sink for C and N utilization. However, whether this process depends on N absorption is unknown. Here, maize seedlings were grown hydroponically with three N forms (NO3- only, 75/25 NO3-/NH4+and NH4+ only). The study results suggested that maize growth rate and N content of shoots under mixed N supply was little different to that under sole NO3- supply at 0-3 d, but was higher than under sole NO3- supply at 6-9 d. 15N influx rate under mixed N supply was greater than under sole NO3- or NH4+ supply at 6-9 d, although NO3- and NH4+ influx under mixed N supply were reduced compared to sole NO3- and NH4+ supply, respectively. qRT-PCR determination suggested that the increased N absorption under mixed N supply may be related to the higher expression of NO3- transporters in roots, such as ZmNRT1.1A, ZmNRT1.1B, ZmNRT1.1C, ZmNRT1.2 and ZmNRT1.3, or NH4+ absorption transporters, such as ZmAMT1.1A, especially the latter. Furthermore, plants had higher nitrate reductase (NR) glutamine synthase (GS) activity and amino acid content under mixed N supply than when under sole NO3- supply. The experiments with inhibitors of NR reductase and GS synthase further confirmed that N assimilation ability under mixed N supply was necessary to promote maize growth, especially for the reduction of NO3- by NR reductase. This research suggested that the increased processes of NO3- and NH4+ assimilation by improving N-absorption ability of roots under mixed N supply may be the main driving force to increase maize growth.

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