Selection of goat 8-casein derived ACE-inhibitory peptide SQPK and insights into its effect and regulatory mechanism on the function of endothelial cells

文献类型： 外文期刊

第一作者： Wu, Yulong

作者： Wu, Yulong;Zhang, Jin;Mu, Tong;Zhang, Hong;Cao, Jianxin;Li, Huanhuan;Tang, Honggang;Chen, Lihong;Zhao, Ke;Wu, Yulong;Xu, Xianrong;Liu, Hongyun;Zhao, Ke

作者机构：

关键词： SQPK; ACE inhibitory peptide; Goat 8-casein; Endothelial cells; Transcriptomic profiling

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 253 卷

页码：

收录情况： SCI

摘要： The angiotensin I-converting enzyme (ACE)-inhibitory peptide SQPK was selected by in silico digestion and virtual screening from goat beta-casein, and its effect and regulatory mechanism on function of endothelial cells was further evaluated. The results showed that SQPK exhibited relatively good ACE inhibition capacity (IC50 = 452.7 mu g/mL). Treatment with 25 mu g/mL SQPK for 12 h significantly elevated nitric oxide (NO) production, stimulated eNOS expression (p < 0.05) and affected the transcriptomic profiling of EA. Hy926 cells. In particular, SQPK stimulated the expression of genes encoding inflammatory cytokines (CXCL1/2 and IL6) but depressed encoding mesenchymal markers (FN1 and CNN3). Furthermore, SQPK modified the expression of genes involved in endothelial-to-mesenchymal transition (EndMT). Therefore, the selected peptide SQPK may exert potential protective effects on the function of endothelial cells by inhibiting the EndMT.

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