Peptide extraction from silver carp (Hypophthalmichthys molitrix) scales via enzymatic hydrolysis and membrane filtration

文献类型： 外文期刊

第一作者： Zu, Xiao-Yan

作者： Zu, Xiao-Yan;Huang, Ya-Qi;Zhao, Ya-Jing;Xiong, Guang-Quan;Liao, Tao;Li, Hai-Lan;Zu, Xiao-Yan;Xiong, Guang-Quan;Liao, Tao;Li, Hai-Lan;Zhao, Ya-Jing;Li, Hai-Lan

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关键词： membrane filtration; peptide extraction; response surface methodology; silver carp scales

期刊名称：ITALIAN JOURNAL OF FOOD SCIENCE （ 影响因子：2.6； 五年影响因子：1.6 ）

ISSN： 1120-1770

年卷期： 2023 年 35 卷 2 期

页码：

收录情况： SCI

摘要： In this work, peptides were extracted from Silver carp (SC) scales via protease hydrolysis and separated using two membranes (M1 and M2). The results revealed that the water: SC scale ratios of 50.6 mL/g, alkaline protease 1 (AP1) dose of 2313.6 U/mL, and pH of 8.14 were the optimal hydrolysis conditions, and the peptide yield reached 88.77 +/- 0.32%. The optimal conditions of peptide separation were clarified: the operating pressure of the M1 (M2) was 0.25 (0.4) MPa, the liquid temperature was 30 degrees C, and the operation time was 65 min. In this case, the per-meability of the M1 (M2) reached 91.73 +/- 96% (79.83 +/- 7.23%), and the average molecular weight of the peptides was 758 Da (576 Da). Compared with M1 peptides, M2 peptides contained more acidic and aromatic amino acids exhibiting free-radical scavenging and tyrosinase inhibition properties. It might provide a way to utilize SC scales as a promising material to produce bioactive peptides.

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