Effects of different enzymatic hydrolysis methods on the bioactivity of peptidoglycan in Litopenaeus vannamei

文献类型: 外文期刊

第一作者: Song Xiaoling

作者: Song Xiaoling;Zhang Yue;Wei Song;Huang Jie;Zhang Yue

作者机构:

关键词: peptidoglycan;enzymatic hydrolysis;Litopenaeus vannamei;phenoloxidase;acid phosphatase;resistance to WSSV

期刊名称:CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY ( 影响因子:1.068; 五年影响因子:0.983 )

ISSN: 0254-4059

年卷期: 2013 年 31 卷 2 期

页码:

收录情况: SCI

摘要: The effects of different hydrolysis methods on peptidoglycan (PG) were assessed in terms of their impact on the innate immunity and disease resistance of Pacific white shrimp, Litop enaeus vannamei. PG derived from Bifidobacterium thermophilum was prepared in the laboratory and processed with lysozyme and protease under varying conditions to produce several different PG preparations. A standard shrimp feed was mixed with 0.05% PG preparations to produce a number of experimental diets for shrimp. The composition, concentration, and molecular weight ranges of the soluble PG were analyzed. Serum phenoloxidase and acid phosphatase activity in the shrimp were determined on Days 6-31 of the experiment. The protective activity of the PG preparations was evaluated by exposing shrimp to white spot syndrome virus (WSSV). Data on the composition of the PG preparations indicated that preparations hydrolyzed with lysozyme for 72 h had more low-molecular-weight PG than those treated for 24 h, and hydrolysis by protease enhanced efficiency of hydrolysis compared to lysozyme. SDS-PAGE showed changes in the molecular weight of the soluble PG produced by the different hydrolysis methods. Measurements of serum phenoloxidase and acid phosphatase activity levels in the shrimp indicated that the PG preparations processed with enzymes were superior to the preparation which had not undergone hydrolysis in enhancing the activity of the two serum enzymes. In addition, the preparation containing more low-molecular-weight PG enhanced the resistance of the shrimp to WSSV, whereas no increased resistance was observed for preparations containing less low-molecular-weight PG. These findings suggest that the immunity-enhancing activity of PG is related to its molecular weight and that increasing the quantity of low-molecular-weight PG can fortify the effect of immunity enhancement.

分类号:

  • 相关文献

[1]Georgenia halophila sp nov., a halophilic actinobacterium isolated from a salt lake. Tang, Shu-Kun,Li, Wen-Jun,Tang, Shu-Kun,Li, Wen-Jun,Wang, Yun,Lou, Kai,Lee, Jae-Chan,Park, Dong-Jin,Kim, Chang-Jin.

[2]Nonomuraea wenchangensis sp nov., isolated from mangrove rhizosphere soil. Wang, Fan,Xu, Xiao-Xiong,Qu, Zhi,Wang, Cheng,Lin, Hai-Peng,Xie, Qing-Yi,Ruan, Ji-Sheng,Hong, Kui,Wang, Fan,Sun, Ming.

[3]Presence of prophenoloxidase in the humoral fluid of amphioxus Branchiostoma belcheri tsingtauense. Pang, QX,Zhang, SC,Wang, CF,Shi, XD,Sun, YN.

[4]Density-dependent prophylaxis in crowded Beet Webworm, Loxostege sticticalis (Lepidoptera: Pyralidae) larvae to a parasitoid and a fungal pathogen. Kong, Hailong,Cheng, Yunxia,Luo, Lizhi,Jiang, Xingfu,Zhang, Lei,Sappington, Thomas W.. 2013

[5]Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene (Ha-acp1) from Heterodera avenae. Huang Wen-kun,Long Hai-bo,Peng Huan,He Wen-ting,Peng De-liang. 2014

[6]Characterization of acid phosphatase from the tick Haemaphysalis longicornis. Zhang, Ping,Tian, Zhancheng,Liu, Guangyuan,Xie, Junren,Luo, Jin,Zhang, Liyan,Shen, Hui. 2011

[7]Effect of ginger extract and vinegar on ATP metabolites, IMP-related enzyme activity, reducing sugars and phosphorylated sugars in silver carp during postslaughter storage. Shi, Ce,Cui, Jianyun,Qin, Na,Luo, Yongkang,Lu, Han,Wang, Hang,Shi, Ce,Luo, Yongkang.

[8]Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune. Zhang, Guo-Qing,Chen, Qing-Jun,Sun, Jian,Wang, He-Xiang,Sun, Jian,Wang, He-Xiang,Han, Chun-Hua.

[9]A novel antimicrobial protein isolated from potato (Solanum tuberosum) shares homology with an acid phosphatase. Yuan, FH,Gao, Y,Liang, CG,Xu, J,Zhang, CL,He, LY.

[10]Effects of mycorrhizal fungi on phytate-phosphorus utilization in trifoliate orange (Poncirus trifoliata L. Raf) seedlings. Shu, Bo,Wang, Peng,Xia, Ren-Xue,Shu, Bo,Shu, Bo.

[11]Spatial distribution and expression of intracellular and extracellular acid phosphatases of cluster roots at different developmental stages in white lupin. Tang, Hongliang,Li, Xiaoqing,Zu, Chao,Zhang, Fusuo,Shen, Jianbo,Tang, Hongliang,Zu, Chao.

[12]Characterisation of the phytase gene in trifoliate orange (Poncirus trifoliata (L.) Raf.) seedlings. Shu, Bo,Wang, Peng,Xia, Ren-Xue,Shu, Bo,Shu, Bo.

[13]Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular Proteases. Wu, Ribang,Liu, Dan,Huang, Jiafeng,Zhang, Jiang,Xiao, Xiao,Lei, Ming,Chen, Yuelin,He, Hailun,Chen, Leilei,Chen, Leilei. 2017

[14]High pressure assist-alkali pretreatment of cotton stalk and physiochemical characterization of biomass. Du, Shuang-kui,Zhu, Xinna,Wang, Hua,Zhou, Dayun,Yang, Weihua,Xu, Hongxia.

[15]Enhanced Enzymatic Hydrolysis of Poplar after Combined Dilute NaOH and Fenton Pretreatment. Zhang, Chunyan,Wang, Shanshan,Cui, Zhongyi,Liu, Ping,Pei, Haisheng. 2016

[16]Development of the cuticular membrane and biomechanical properties in Hupingzao (Ziziphus jujuba Mill. 'Hupingzao'). Li, Na,Song, Yuqin,Chen, Yuanyuan,Li, Liulin,Li, Jie,Xue, Xiaofang. 2018

[17]Properties of Klebsiella phage P13 and associated exopolysaccharide depolymerase. Liu Yang,Chai Zihan,Shang Anqi,Mou Haijin,Li Guiyang,Mo Zhaolan. 2014

[18]ENZYMATIC-HYDROLYSIS OF WHEAT-STRAW IRRADIATED BY ELECTRON-BEAM IN PRESENCE OF PERACETIC-ACID SOLUTION. LU, ZX,KUMAKURA, M. 1995

[19]Effect of Ionic Liquid Pretreatment on the Structure and Enzymatic Saccharification of Cassava Stillage Residues. Cui, Lihong,Li, Jihua,Wang, Qinghuang,Wang, Meng. 2014

[20]Preliminary evaluation of five elephant grass cultivars harvested at different time for sugar production. Li, Yuanyuan,Zhang, Yelong,Zheng, Hongbo,Zhang, Hongman,Du, Jian,Huang, He,Wu, Juanzi. 2015

作者其他论文 更多>>