Major histocompatibility complex class IIA and IIB genes of Nile tilapia Oreochromis niloticus: Genomic structure, molecular polymorphism and expression patterns

文献类型： 外文期刊

第一作者： Pang, Ji-cai

作者： Pang, Ji-cai;Gao, Feng-ying;Lu, Mai-xin;Ye, Xing;Zhu, Hua-ping;Ke, Xiao-li;Gao, Feng-ying;Lu, Mai-xin;Ye, Xing;Zhu, Hua-ping;Ke, Xiao-li;Pang, Ji-cai

作者机构：

关键词： Nile tilapia (Oreochromis niloticus);Major histocompatibility complex class II (MHC class II);Genomic structure;Molecular polymorphism;Expression

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2013 年 34 卷 2 期

页码：

收录情况： SCI

摘要： Major histocompatibility complex (MHC) is a large genomic region characterized by extremely high polymorphism, and it plays an important role in the immune response of vertebrates. In the present study, we isolated MHC class II genes from Nile tilapia in order to investigate the immune mechanism in tilapia and develop better strategies for disease prevention. Moreover, we cloned the full-length cDNA sequences of MHC IIA and IIB from Nile tilapia by the RACE approach. In addition, the genomic structure, molecular polymorphism and expression patterns of MHC II genes in Nile tilapia were also examined. Compared with that of other teleosts, Nile tilapia MHC class HA contained four exons and three introns. The deduced amino acid sequence of the MHC IIA molecule shared 25.4-64.5% similarity with those of other teleosts and mammals. Six exons and five introns were identified from Nile tilapia MHC IIB, and the deduced amino acid sequence shared 26.9-74.7% similarity with those of other teleosts and mammals. All the characteristic features of MHC class II chain structure could be identified in the deduced sequences of MHC IIA and JIB molecules, including the leader peptide, alpha 1/beta 1 and alpha 2/beta 2 domains, connecting peptide and transmembrane and cytoplasmic regions, as well as conserved cysteines and N-glycosylation site. A total of 12 MHC IIA alleles were identified from six individuals. Four alleles originating from a single individual suggested that at least four MHC IIA loci existed. Moreover, 10 MHC JIB alleles were identified, among which four were detected in a single individual, suggesting that at least four MHC IIB loci existed. The expression of MHC IIA and IIB at the mRNA level in 10 types of normal tissues was determined using quantitative real-time PCR analysis. The highest expression level was detected in stomach and gill, whereas the lowest expression was detected in muscle and brain. Furthermore, MHC IIA and JIB were probably two candidate immune molecules involved in the resistance against streptococcosis, because their expression was significantly up-regulated in gill, kidney, intestine and spleen after the intraperitoneal injection of Streptococcus agalactiae. (C) 2012 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Isolation and characterization of Toll-like receptor 21 and 22 genes from Nile tilapia, Oreochromis niloticus (Linnaeus). Pang, Ji-cai,Gao, Feng-ying,Zhao, Jin-liang,Pang, Ji-cai,Gao, Feng-ying,Wang, Miao,Lu, Mai-xin,Pang, Ji-cai,Gao, Feng-ying,Wang, Miao,Lu, Mai-xin.

[2]Molecular polymorphism of chlorpyrifos-degrading strains in soil treated with controlled and non-controlled release formulations of chlorpyrifos. Chen, Liezhong,Li, Yanli,Wang, Ting,Chen, Liezhong,Yu, Yunlong.

[3]Molecular characterization, expression and functional analysis of NOD1, NOD2 and NLRC3 in Nile tilapia (Oreochromis niloticus). Gao, Feng-ying,Yang, Xian-le,Gao, Feng-ying,Pang, Ji-cai,Lu, Mai-xin,Zhu, Hua-ping,Ke, Xiao-li,Liu, Zhi-gang,Cao, Jian-meng,Wang, Miao,Gao, Feng-ying,Pang, Ji-cai,Lu, Mai-xin,Zhu, Hua-ping,Ke, Xiao-li,Liu, Zhi-gang,Cao, Jian-meng,Wang, Miao. 2018

[4]Species diversity defends against the invasion of Nile tilapia (Oreochromis niloticus). Gu, Dang E.,Luo, Du,Xu, Meng,Ma, Guang M.,Mu, Xi D.,Luo, Jian R.,Hu, Yin C.,Ma, Guang M.. 2014

[5]Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.). Lv, Geng-Yin,Guo, Xiao-Guang,Xie, Li-Ping,Xie, Chang-Gen,Zhang, Xiao-Hong,Yang, Yuan,Xiao, Lei,Tang, Yu-Ying,Guo, Ai-Guang,Xu, Hong,Xie, Chang-Gen,Guo, Ai-Guang,Xu, Hong,Pan, Xing-Lai. 2017

[6]Molecular cloning, expression and characterization of bovine UQCC and its association with body measurement traits. Liu, Yongfeng,Zan, Linsen,Zhao, Shuanping,Liu, Yongfeng,Huang, Honggang,Li, Yong,Tang, Zhonglin,Yang, Shulin,Li, Kui,Zan, Linsen. 2010

[7]MOLECULAR CHARACTERIZATION, POLYMORPHISM AND EXPRESSION ANALYSIS OF SWAMP EEL MAJOR HISTOCOMPATIBILITY COMPLEX CLASS II B GENE, AFTER INFECTION BY AEROMONAS HYDROPHILIA. Li, W.,Sun, W. X.,Hu, J. F.,Hong, D. W.,Li, W.,Chen, S. L.. 2014

[8]Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA and IIB genes of half-smooth tongue sole (Cynoglossus semilaevis). Xu, Tian-jun,Chen, Song-lin,Ji, Xiang-shan,Sha, Zhen-xia,Xu, Tian-jun.

[9]Genomic organization and promoter characterization of FcCTL, a C-type lectin-like protein in Fenneropenaeus chinensis. Lai, XiaoFang,Gao, Huan,Yan, Binlun,Lai, XiaoFang,Gao, Huan,Kong, Jie,Wang, QingYin,Meng, XianHong,Chen, Baiyao.

[10]Characterization of the porcine p65 subunit of NF-kappa B and its association with virus antibody levels. Cao, Jiyue,Li, Hegang,Ma, Guojian,Gui, Duan,Zhao, Shuanping,Wang, Pan,Ruan, Jinxue,Yang, Shulin,Li, Kui,He, Kongwang,Wang, Xuemin. 2011

[11]Characterization of porcine MMP-2 and its association with immune traits. Huang, Honggang,Zhao, Weimin,Tang, Zhonglin,Yang, Shulin,Cui, Wentao,Mu, Yulian,Chu, Mingxing,Li, Kui,Zhao, Weimin,Zhao, Shuhong,Wu, Zhengfang. 2009

[12]Structural Characterization and Association of Ovine Dickkopf-1 Gene with Wool Production and Quality Traits in Chinese Merino. Mu, Fang,Rong, Enguang,Jing, Yang,Ma, Guangwei,Yan, Xiaohong,Wang, Zhipeng,Li, Yumao,Li, Hui,Wang, Ning,Yang, Hua. 2017

[13]Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization. Shi, Yan,Yin, Jing-Kui,Zhu, Xin-Ping,Chen, Kun-Ci,Pan, De-Bo,Zhao, Zhe,Zhu, Xin-Ping,Gui, Jian-Fang.

[14]Molecular cloning and characterization of S-adenosylmethionine decarboxylase gene in rubber tree (Hevea brasiliensis). Zhao, Manman,Liu, Hui,Deng, Zhi,Chen, Jiangshu,Yang, Hong,Li, Dejun,Zhao, Manman,Li, Huiping,Xia, Zhihui.

[15]Genomic structure, characterization and expression analysis of a manganese superoxide dismutase from pearl oyster Pinctada fucata. Zhang, Dianchang,Cui, Shuge,Guo, Huayang,Jiang, Shigui,Zhang, Dianchang,Jiang, Shigui.

[16]Molecular characterization of the porcine STAT4 and STAT6 genes. Huang, Jing,Ma, Guojian,Zhu, Mengjin,Zhao, Shu-Hong,Huang, Jing,Ma, Guojian,Zhu, Mengjin,Zhao, Shu-Hong,Huang, Jing,Pan, Jianzhi,Zhang, Wenchang.

[17]Renaturation and purification of ApxII toxin of Actinobacillus pleuropneumoniae. Wang, Chunlai,Liu, Siguo,Peng, Yonggang,Shao, Meili,Wang, Yong,Gong, Qiang,Chang, Yuehong,Liu, Jiandong,Liu, Huifang,Liu, Di,Kong, Xiangang.

[18]Expression of Prolactin Receptor mRNA after Melatonin Manipulated in Cashmere Goats Skin during Cashmere Growth. Zhang, Wei,Zhu, Xiaoping,Jia, Zhihai,Yue, Chunwang,Kong, Xianghao,Du, Lixin.

[19]Isolation and characterization of a cDNA encoding a papain-like cysteine protease from alfalfa. Yan, Longfeng,Han, Jianguo,Sun, Yan,Yan, Longfeng,Yang, Qingchuan,Kang, Junmei,Liu, Zhipeng,Wu, Mingsheng.

[20]Effects of age and strain on the expression of leptin receptor, neuropeptide Y and pro-opiomelanocortin in the hypothalamus of young chickens. Huang, J. X.,Lu, L.,Luo, X. G.,Liu, B.,Huang, J. X.,Lu, L.,Luo, X. G.,Liu, B.,Huang, J. X.,Xi, L..

作者其他论文 更多>>

微信小程序