Picomol Assay of Nitric Oxide Screening in Biological Samples by Derivatization Combined with High-throughput Microplate Format
文献类型: 外文期刊
第一作者: Zhang, Xiaoling
作者: Zhang, Xiaoling;Yang, Qiao
作者机构:
关键词: Nitric oxide;Biological samples;High-throughput;Microplate format;Derivatization
期刊名称:APPLIED MATERIALS AND TECHNOLOGIES FOR MODERN MANUFACTURING, PTS 1-4
ISSN: 1660-9336
年卷期: 2013 年 423-426 卷
页码:
收录情况: SCI
摘要: Nitric oxide (NO) is a critical intra- and extra-cellular signaling molecule that mediates a broad variety of physiologic and pathophysiologic events, and has prompted rapid growth of investigations in investigating its physiology and generation mechanism of corresponding diseases. In this paper, a picomol assay of NO screening method using fluorescent derivatization combined with high-throughout microplate format has been developed and successfully applied to the determination of NO in biological samples. The satisfied detection limit and recovery of the proposed method demonstrates that it can be competent for the sensitive determination of NO in extremely small volume of biological sample.
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