Desalination of Antioxidant Peptides from Pinctada Fucata Using C18 Chromatographic Column

文献类型： 外文期刊

第一作者： Wu, Yanyan

作者： Wu, Yanyan;Wang, Jing;Li, Laihao;Yang, Xianqing;Hu, Xiao

作者机构：

关键词： Pinctada Fucata;antioxidant peptides;C18 chromatographic column;desalination technology

期刊名称：ADVANCES IN CHEMICAL ENGINEERING III, PTS 1-4

ISSN： 1022-6680

年卷期： 2013 年 781-784 卷

页码：

收录情况： SCI

摘要： Antioxidant peptides crude product from Pinctada Fucata desalination technology by C18 chromatographic column was studied. The desalination rate and the antioxidant activity were used to analyze the effect of desalination conditions. The results show that the optimum conditions for desalting are loading 2ml of sample with concentration of 100 mg/ml, flow rate of 2 ml/min, 0.1% TFA as mobile phase with pH 6.5 in 0-4.5min and 80% methanol in 4.5-30min. Under these conditions, the desalination rate is 68.75% and the peptide recovery rate is 76.53%. The inhibitory content (IC50) expressed by antioxidant peptides is 11.421mg/ml in the system of DPPH radical.

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