Study of the occurrence of toxic alkaloids in forage grass by liquid chromatography tandem mass spectrometry

文献类型： 外文期刊

第一作者： Qie, Mengjie

作者： Qie, Mengjie;Li, Shuangyue;Yang, Shuming;Zhao, Yan;Qie, Mengjie;Li, Shuangyue;Yang, Shuming;Zhao, Yan;Guo, Chuntao

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关键词： Forage grass; Feed; Ergot alkaloids; Pyrrolizidine alkaloids; Tropane alkaloids; LC-MS; MS

期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.759； 五年影响因子：4.302 ）

ISSN： 0021-9673

年卷期： 2021 年 1654 卷

页码：

收录情况： SCI

摘要： The toxic alkaloids in forage grass present a serious health hazard to humans and livestock, especially ergot alkaloids (EAs), pyrrolizidine alkaloids (PAs) and tropane alkaloids (TAs). Hence, there is a need for a simultaneous method that allows these dangerous plant toxins to be determined. A simple and effective method was developed to determine fifteen toxic alkaloids (EAs, PAs and TAs) in forage grass using the QuEChERS method and liquid chromatography tandem mass spectrometry (LC-MS/MS). The developed method was validated with average recoveries ranging from 63.10 to 102.10%, and relative standard deviations lower than or equal to 6.39% were obtained. Good linearity over the concentration range of 10-600 mu g/kg dry matter (DM) was observed for the target alkaloids. The determination coefficients R 2 calculated for each of the matrix calibration curves were greater than 0.99. The limits of detection and quantification were 5 mu g/kg DM and 10 mu g/kg DM, respectively. The reproducibility of the method was verified in three laboratories: all of the mean recoveries of 15 alkaloids were higher than 60%, and the relative standard deviations in alkaloids between laboratories were all less than 14.24%. The proposed method was applied to analyse 134 forage grass samples from the meadow steppe of Inner Mongolia to monitor toxic alkaloids. A significant difference in the frequency of contamination was found between different herbage species and different regions. (c) 2021 Elsevier B.V. All rights reserved.

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