Study of Ultrasonic-freeze-thaw-cycle Assisted Extraction of Polysaccharide and Phycobiliprotein from Gracilaria lemaneiformis
文献类型: 外文期刊
第一作者: Liu, Mingqiu
作者: Liu, Mingqiu;Yang, Xianqing;Qi, Bo;Li, Laihao;Deng, Jianchao;Hu, Xiao
作者机构:
关键词: ultrasonic-freeze-thaw-cycle assisted extraction;polysaccharide;phycobiliprotein;Gracilaria lemaneiformis
期刊名称:ADVANCES IN CHEMICAL ENGINEERING III, PTS 1-4
ISSN: 1022-6680
年卷期: 2013 年 781-784 卷
页码:
收录情况: SCI
摘要: Ultrasonic-freeze-thaw-cycle assisted extraction was used to synchronously extract polysaccharide and phycobiliprotein from Gracilaria lemaneiformis. Firstly, different solvents(deionized water, sodium hydroxide, phosphate buffer solution (PBS) and calcium chloride solution) were screened, and PBS (1 mM, pH 6.8) was considered as the best solvent for its OD565 value (0.086+/-0.0003) and OD595 value (0.471+/-0.002), which was significantly higher than any other solvents (p<0.05). Moreover, an L-9 (3)(4) orthogonal test was carried out to optimize extraction conditions, based on the single-factor test. The results indicated that the optimized conditions were fixed as follows: the ratio of solid to liquid 1:25 (w/v), 2 times of freeze-thaw cycles, ultrasound power 320 W and ultrasound time 10 min, which obtained the optimum yield and purity of polysaccharide and phycobiliprotein, 30.54%, 1.36% and 0.296, respectively. As a result, ultrasonic-freeze-thaw-cycle assisted extraction is considered to be an effective, economic and eco-environmental method to extract polysaccharide and phycobiliprotein.
分类号:
- 相关文献
作者其他论文 更多>>
-
Raw meat 3D laser scanning imaging: Optimized by adaptive contour unit
作者:Bu, Lingping;Tian, Huixin;Hu, Xiaojia;Qi, Bo;Wang, Zhize;Hu, Jingfang;Zhang, Chunhui;Zhang, Dequan;Wei, Wensong;Bu, Lingping;Gao, Guowei;Qi, Bo;Hu, Jingfang;Qiao, Zhen;Tian, Huixin;Qiao, Zhen;Hu, Xiaojia;Zhang, Chunhui;Zhang, Dequan;Wei, Wensong;Gao, Guowei;Hu, Jingfang;Wang, Zhize
关键词:3D scanning imaging; Contour visualization; Adaptive shaping; Hole compensation; Meat identification; Volume estimation
-
Predicting Soil Organic Carbon Content by Combining Unmanned Aerial Vehicle Multispectral Images and Machine Learning Algorithms
作者:Qi, Guanghui;Lu, Hangyu;Hu, Xiao;Zang, Yulong;Li, Xinju;Zhang, Jiguang
关键词:Soil organic carbon (SOC); Unmanned aerial vehicle (UAV); Red-edge; Modified spectral indices; Machine learning (ML)
-
Flupyradifurone Exhibits Greater Toxicity to the Asian Bumblebee Bombus lantschouensis Compared to the European Bumblebee Bombus terrestris
作者:Jie, Chunting;Zhang, Hong;Zhou, Ziyu;Han, Bo;Guo, Baodi;Guo, Yi;Hu, Xiao;Iqbal, Shahid;Wei, Bingshuai;Huang, Jiaxing;Dai, Pingli;An, Jiandong;Miao, Zhengying
关键词:bumblebee; acute toxicity; chronic toxicity; risk assessment
-
Effects of Sodium Hypochlorite Rinsing on Tilapia Storage: An Investigation Based on Muscle Quality and Tissue Protease Activity
作者:Fu, Zirui;Hao, Shuxian;Xiang, Huan;Li, Chunsheng;Cen, Jianwei;Wei, Ya;Chen, Shengjun;Zhao, Yongqiang;Hu, Xiao;Yan, Yuhong;Huang, Hui;Fu, Zirui;Hao, Shuxian;Xiang, Huan;Li, Chunsheng;Cen, Jianwei;Wei, Ya;Chen, Shengjun;Zhao, Yongqiang;Hu, Xiao;Huang, Hui;Li, Jun
关键词:tilapia storage; endogenous enzyme activity; hypochlorite rinsing; bacterial reduction; quality characteristics; muscle quality
-
A Novel Strategy for the Detection of Semicarbazide in Crustaceans by Modified QuEChERS Coupled with Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry
作者:Wu, Siyuan;Chen, Shengjun;Wu, Siyuan;Feng, Yang;Chen, Shengjun;Zhao, Yongqiang;Li, Chunsheng;Deng, Jianchao;Wang, Di;Feng, Yang;Chen, Shengjun;Zhao, Yongqiang;Li, Chunsheng;Deng, Jianchao;Wang, Di
关键词:semicarbazide; crustaceans; shrimp; QuEChERS; HILIC-MS/MS;
Macrobrachium rosenbergii ; nitrofurazone; kinetic model -
FPAW from Trachinotus ovatus Attenuates Potassium-Oxonate-Induced Hyperuricemia in Mice via Xanthine Oxidase Inhibition and Gut Microbiota Modulation: Molecular Insights and In Vivo Efficacy
作者:Xiang, Huan;Sun-Waterhouse, Dongxiao;Hu, Xiao;Hou, Mengfan;Chen, Shengjun;Wu, Yanyan;Zhao, Yongqiang;Wang, Yueqi;Xiang, Huan;Hu, Xiao;Chen, Shengjun;Wu, Yanyan;Zhao, Yongqiang;Wang, Yueqi;Sun-Waterhouse, Dongxiao
关键词:hyperuricemia; XOD inhibitory; gut microbiota; short-chain fatty acids; molecular docking
-
Detection of amino thiols from fish samples by stable isotope chemical labelling coupled with ultra-performance liquid chromatography-tandem mass spectrometry
作者:Wu, Siyuan;Feng, Yang;Chen, Shengjun;Zhao, Yongqiang;Deng, Jianchao;Wang, Di;Feng, Yang;Chen, Shengjun;Zhao, Yongqiang;Deng, Jianchao;Wang, Di;Wu, Siyuan;Chen, Shengjun;Zhao, Yongqiang;Deng, Jianchao
关键词:Amino thiols; Liquid extraction; Iodoacetamide; Stable isotope labeling; UPLC-MS; Transportation stress