Isolation and characterization of polymorphic microsatellite markers from Coilia ectenes

文献类型： 外文期刊

第一作者： Rong, X. J.

作者： Rong, X. J.;Wang, Q. Y.;Rong, X. J.;Xu, Y. J.;Wang, Q. Y.;Liao, M. J.;Liu, X. Z.;Pan, C. Y.;Zhang, Z.;Wang, Y. G.

作者机构：

关键词： Coilia ectenes;Microsatellite marker;Genetic diversity

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2013 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Coilia ectenes (Jordan and Seale 1905) is an important anadromous species that is an important resource at risk of extinction because of over-fishing, pollution, and coastal construction. To evaluate the genetic diversity of C. ectenes for use in breeding programs, elite microsatellite-enriched libraries were constructed and novel microsatellite markers were developed, and applied to genetically detect wild populations. Out of 92 randomly selected and sequenced clones, 89 contained a CA or GA repeat motif. Twenty-two pairs of primers were designed to investigate the polymorphism and genetic structure of a wild population collected from the Yellow River estuary, China. It was found that 2 loci were monomorphic and 20 loci were polymorphic. The number of alleles per polymorphic loci ranged from 3 to 13, with an average of 7.9. The expected heterozygosity per locus ranged from 0.05 to 0.89, with an average of 0.68. The isolated polymorphic markers are expected to be of use in future genetic breeding programs for C. ectenes, and in the assessment of genetic variation within this species.

分类号：

  • 相关文献

[1]Genetic diversity and demographical history of Coilia ectenes (Clupeiformes: Engraulidae) inferred from the complete control region sequences of mitochondrial DNA. Ma, Chunyan,Cheng, Qiqun,Zhang, Qingyi.

[2]PCR-RFLP analysis of cytochrome b gene does not support Coilia ectenes taihuensis being a subspecies of Coilia ectenes. Cheng, QQ,Lu, DR.

[3]Genetic variation of Coilia ectenes (Clupeiformes: Engraulidae) revealed by the complete cytochrome b sequences of mitochondrial DNA. Ma, Chunyan,Cheng, Qiqun,Zhang, Qingyi,Zhuang, Ping,Ma, Chunyan,Zhao, Yunlong,Zhang, Qingyi.

[4]Evaluation of the genetic diversity and population structure of five indigenous and one introduced Chinese goose breeds using microsatellite markers. Li, Jinjun,Yuan, Qingyuan,Shen, Junda,Tao, Zhengrong,Li, Guoqing,Tian, Yong,Wang, Deqian,Chen, Li,Lui, Lizhi. 2012

[5]Genetic characterization of three breeds of high royal jelly producing honeybee (Apis mellifera ligustica) in China. Yin, Ling,Ji, Ting,Chen, Guohong,Peng, Wenjun. 2011

[6]Development of microsatellite markers and their utilization in genetic diversity analysis of cultivated and wild populations of the mud carp (Cirrhina molitorella). Yang, Cheng,Sun, Xiaowen,Yang, Cheng,Zhu, Xinping. 2008

[7]Genetic diversity analysis of oriental river prawn, Macrobrachium nipponense, in Yellow River using microsatellite marker. Qiao, H.,Jiang, S. F.,Sun, M.,Gong, Y. S.,Xiong, Y. W.,Jin, S. B.,Fu, H. T.,Lv, D.. 2013

[8]Evaluation of genetic identity and variation in cultivars of Pyrus pyrifolia (Burm.f.) Nakai from China using microsatellite markers. Cao, Y.,Yang, J.,Zhang, S.,Cao, Y.,Tian, L.,Gao, Y..

[9]Genetic diversity a population structure analysis of a accessions in the Chinese cowpea [Vigna unguiculata (L.) Walp.] germplasm collection. Chen, Honglin,Hu, Liangliang,Wang, Lixia,Wang, Suhua,Cheng, Xuzhen,Chen, Hong,Wang, Ming Li. 2017

[10]NUCLEAR AND CHLOROPLAST SSR MARKERS IN PAEONIA DELAVAYI (PAEONIACEAE) AND CROSS-SPECIES AMPLIFICATION IN P. LUDLOWII. Zhang, Jin-Mei,Liu, Jing,Sun, Hai-Lu,Yu, Jing,Wang, Jian-Xiu,Zhou, Shi-Liang,Zhang, Jin-Mei,Zhang, Jin-Mei,Liu, Jing,Sun, Hai-Lu,Yu, Jing,Wang, Jian-Xiu. 2011

[11]Genetics driven interventions for ex situ conservation of red junglefowl (Gallus gallus murghi) populations in India. Mukesh,Fernandes, Merwyn,Sathyakumar, Sambandam,Mukesh,Han, Jianlin.

[12]Identification and characterization of microsatellite markers from Musa balbisiana. Feng, Su-ping,Wu, Yao-ting,Wang, Jing-yi,Wang, Jing-yi,Huang, Bing-zhi,Chen, Ye-yuan.

[13]Genetic Diversity of Chinese Indigenous Pig Breeds in Shandong Province Using Microsatellite Markers. Wang, J. Y.,Guo, J. F.,Hu, H. M.,Lin, H. C.,Wang, Cheng,Zhang, Yin,Wu, Y.,Wang, J. Y.,Zhang, Q..

[14]Genetic analysis of Phytophthora sojae populations in Fujian, China. Wu, M.,Li, B.,Liu, P.,Weng, Q.,Chen, Q.,Wu, M.,Zhan, J.,Chen, Q..

[15]Morphological differences between close populations discernible by multivariate analysis: A case study of genus Coilia (Teleostei : Clupeiforms). Cheng, QQ,Lu, DR,Ma, L. 2005

[16]Development of thirty-four novel polymorphic microsatellite markers in Coilia ectenes (Clupeiformes: Engraulidae) and cross-species amplification in two closely related taxa. Chen, Fang,Zeng, Liyan,Cheng, Qiqun,Chen, Fang. 2012

[17]In-depth transcriptome analysis of Coilia ectenes, an important fish resource in the Yangtze River: de novo assembly, gene annotation. Shen, Huaishun,Gu, Ruobo,Xu, Gangchun,Xu, Pao,Nie, Zijuan,Shen, Huaishun,Xu, Pao,Hu, Yacheng.

[18]Reconstructing habitat use of Coilia mystus and Coilia ectenes of the Yangtze River estuary, and of Coilia ectenes of Taihu Lake, based on otolith strontium and calcium. Yang, J.,Arai, T.,Liu, H.,Miyazaki, N.,Tsukamoto, K.. 2006

[19]The complete mitochondrial genome sequence of Coilia ectenes (Clupeiformes: Engraulidae). Qiao, Huiying,Cheng, Qiqun,Chen, Ying,Chen, Wenming,Zhu, Yuxia,Qiao, Huiying,Chen, Ying,Chen, Wenming,Zhu, Yuxia.

[20]Genetic structure and differentiation of four lake populations of Coilia ectenes (Clupeiformes: Engraulidae) based on mtDNA control region sequences. Cheng, Qiqun,Zhang, Qingyi,Ma, Chunyan,Guan, Weibing.

作者其他论文 更多>>

微信小程序