Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)

文献类型： 外文期刊

第一作者： Gao, Zhengquan

作者： Gao, Zhengquan;Meng, Chunxiao;Gao, Hongzheng;Su, Yuanfeng;Wang, Yuanyuan;Zhao, Yuren;Zhang, Xiaowen;Xu, Dong;Ye, Naihao

作者机构：

关键词： Haematococcus pluvialis;astaxanthin;2, 4-Epibrassinolide (EBR);carotenoid genes;real-time fluorescence quantitative PCR (qRT-PCR)

期刊名称：BIOLOGICAL RESEARCH （ 影响因子：5.612； 五年影响因子：4.781 ）

ISSN： 0716-9760

年卷期： 2013 年 46 卷 2 期

页码：

收录情况： SCI

摘要： The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5- fold up-regulation) than on pay, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on ipi-1 and psy. Furthermore, astaxanthin biosynthesis under EBR was up-regulated mainly by ipi-1 and psy at the post-transcriptional level, pds, lyc, crtR-B, bkt and crtO at the transcriptional level and ipi-2 at both levels.

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