Interaction of C/EBP beta with SMAD2 and SMAD4 genes induces the formation of lipid droplets in bovine myoblasts

文献类型： 外文期刊

第一作者： Wei, Dawei

作者： Wei, Dawei;Zhang, Guijie;Zhang, Le;Raza, Sayed Haidar Abbas;Raza, Sayed Haidar Abbas;Zhang, Jiupan;Liang, Xiaojun;Juan, Zhao;Al-Amrah, Hadba;Al Abdulmonem, Waleed;Alharbi, Yousef Mesfer

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关键词： SMAD2; SMAD4; C/EBP beta; Transcriptional regulation; Myoblast

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：2.9； 五年影响因子：3.3 ）

ISSN： 1438-793X

年卷期： 2023 年 23 卷 2 期

页码：

收录情况： SCI

摘要： As a key component of Transforming growth factor-beta (TGF-beta) pathway, Smad2 has many crucial roles in a variety of cellular processes, but it cannot bind DNA without complex formation with Smad4. In the present study, the molecular mechanism in the progress of myogenesis underlying transcriptional regulation of SMAD2 and SMAD4 had been clarified. The result showed the inhibition between SMAD2 and SMAD4, which promotes and inhibits bovine myoblast differentiation, respectively. Further, the characterization of promoter region of SMAD2 and SMAD4 was analyzed, and identified C/EBP beta directly bound to the core region of both SMAD2 and SMAD4 genes promoter and stimulated the transcriptional activity. However, C/EBP beta has lower expression in myoblasts which plays vital function in the transcriptional networks controlling adipogenesis, while the overexpression of C/EBP beta gene in myoblasts significantly increased SMAD2 and SMAD4 gene expression, induced the formation of lipid droplet in bovine myoblasts, and promoted the expression of adipogenesis-specific genes. Collectively, our results showed that C/EBP beta may play an important role in the trans-differentiation and dynamic equilibrium of myoblasts into adipocyte cells via promoting an increase in SMAD2 and SMAD4 gene levels. These results will provide an important basis for further understanding of the TGF beta pathway and C/EBP beta gene during myogenic differentiation.

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