Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)

文献类型： 外文期刊

第一作者： Shi, Yan

作者： Shi, Yan;Zhu, Xinping;Chen, Kunci;Zhao, Zhe;Zhang, Qiya

作者机构：

关键词： GRIM-19;Epinephelus coioides;Tissue distribution;Subcellular localization;Overexpression

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2013 年 34 卷 1 期

页码：

收录情况： SCI

摘要： GRIM-19 is a nuclear encoded subunit of complex I that has been implicated in apoptosis. The protein participates in multiple functions including the innate immune response. GRIM-19 has been studied in humans and other mammals; however, fish GRIM-19 has not been well characterized. In this study, a new GRIM-19 gene, EcGRIM-19, was isolated from the orange-spotted grouper (Epinephelus coioides) cDNA library, which was constructed following LPS treatment. EcGRIM-19 is a 582-bp gene that encodes a 144-amino acid protein. The gene is a true ortholog of mammalian GRIM-19. EcGRIM-19 exhibits ubiquitous and constitutive expression in the different tissues of the orange-spotted grouper. The expression levels of EcGRIM-19 are altered in the gill, spleen, kidney and liver after induction with LPS. The subcellular localization analysis demonstrated that the EcGRIM-19 protein is localized predominantly in the mitochondria. In addition, amino acids 30-50 of the protein are responsible for the mitochondrial localization of EcGRIM-19. The caspase assay demonstrated that the overexpression of GRIM-19 enhanced the cellular sensitivity to interferon(IFN)-beta- and retinoic acid (RA)-induced death in HeLa cells. The data presented in this study are important for further understanding the EcGRIM-19 gene function in fish. (c) 2012 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Characterization and Functional Analysis of Voltage-Dependent Anion Channel 1 (VDAC1) from Orange-Spotted Grouper (Epinephelus coioides). Shi, Yan,Hong, Xiaoyou,Chen, Kunci,Zhu, Xinping,Zhao, Zhe.

[2]Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization. Shi, Yan,Yin, Jing-Kui,Zhu, Xin-Ping,Chen, Kun-Ci,Pan, De-Bo,Zhao, Zhe,Zhu, Xin-Ping,Gui, Jian-Fang.

[3]Isolation and functional characterization of salt-stress induced RCI2-like genes from Medicago sativa and Medicago truncatula. Long, Ruicai,Zhang, Fan,Li, Zhenyi,Cong, Lili,Kang, Junmei,Zhang, Tiejun,Yang, Qingchuan,Li, Mingna,Sun, Yan,Zhao, Zhongxiang,Zhao, Zhongxiang.

[4]Molecular cloning and multifunctional characterization of GRIM-19 (gene associated with retinoid-interferon-induced mortality 19) homologue from turbot (Scophthalmus maximus). Wang, Na,Wang, Tianzi,Chen, Songlin,Wang, Xianli,Yang, Changgeng,Zhao, Xiaojie,Zhang, Yuxi.

[5]Growth, feed intake and immune responses of orange-spotted grouper (Epinephelus coioides) exposed to low infectious doses of ectoparasite (Cryptocaryon irritans). Yin, Fei,Sun, Peng,Shi, Zhao-Hong,Gao, Quan-Xin,Peng, Shi-Ming,Dan, Xue-Ming,Li, An-Xing. 2014

[6]Polygenic expression of somatostatin in orange-spotted grouper (Epinephelus coioides): Molecular cloning and distribution of the mRNAs encoding three somatostatin precursors. Ying, Y,Li, WS,Lin, HR. 2005

[7]Ontogenetic Development of Digestive Enzymes in Orange-Spotted Grouper (Epinephelus coioides, Hamilton, 1822) Larvae. Guo, Huayang,Li, Jianzhu,Ma, Zhenhua,Zhang, Jiasong,Zhen, Panlong,Cheng, Dachuan,Guo, Huayang. 2016

[8]Molecular characterization and functional analysis of TRAF6 in orange-spotted grouper (Epinephelus coioides). Li, Yan-Wei,Li, Xia,Xiao, Xi-Xi,Li, An-Xing,Zhao, Fei,Luo, Xiao-Chun,Dan, Xue-Ming.

[9]Mass Mortality of Cage-cultured Orange-spotted Grouper Epinephelus coioides Associated with Renal Sphaerosporosis Caused by Sphaerospora epinepheli in South China Sea. Xu, Li-Wen,Feng, Juan,Zhang, Jing-Yong,Wang, Jian-Guo.

[10]Integrated transcriptome, proteome and physiology analysis of Epinephelus coioides after exposure to copper nanoparticles or copper sulfate. Wang, Tao,Han, Shiqun,Yan, Shaohua,Wang, Tao,Long, Xiaohua,Chen, Xiaoyan,Liu, Yuanrui,Liu, Zhaopu,Yan, Shaohua.

[11]Distribution and Metabolism of Bt-Cry1Ac Toxin in Tissues and Organs of the Cotton Bollworm, Helicoverpa armigera. Li, Yunhe,Xiao, Yutao,Ali, Abid,Dhiloo, Khalid Hussain,Chen, Wenbo,Wu, Kongming,Ali, Abid,Dhiloo, Khalid Hussain. 2016

[12]Tissue distribution and expression of signaling lymphocyte activation molecule receptor to peste des petits ruminant virus in goats detected by real-time PCR. Meng, Xuelian,Dou, Yongxi,Zhai, Junjun,Zhang, Hairui,Yan, Fengchao,Shi, Xiaoni,Luo, Xuelong,Li, Hui,Cai, Xuepeng. 2011

[13]Occurrence and dietary exposure assessment of PFOS and PFOA in cultured Trachinotus ovatus in China. Meng, Di,Qian, Yongzhong,Meng, Di,Han, Gang,Guo, Mengmeng. 2017

[14]Molecular characterization and expression of a novel big defensin (Sb-BDef1) from ark shell, Scapharca broughtonii. Li, Meng,Zhu, Ling,Zhou, Chun-ya,Sun, Shan,Fan, Yan-jun,Zhuang, Zhi-meng,Li, Meng,Zhou, Chun-ya. 2012

[15]A novel serine protease with clip domain from scallop Chlamys farreri. Zhu, Ling,Song, Linsheng,Zhao, Jiangmin,Li, Chenghua,Xu, Wei,Zhu, Ling,Mao, Yuze. 2008

[16]Ontogeny expression of ghrelin, neuropeptide Y and cholecystokinin in blunt snout bream, Megalobrama amblycephala. Ping, H. -C.,Feng, K.,Zhang, G. -R.,Wei, K. -J.,Wang, W. -M.,Zou, G. -W.. 2014

[17]IDENTIFICATION AND CHARACTERIZATION OF TWO SENSORY NEURON MEMBRANE PROTEINS FROM Cnaphalocrocis medinalis (LEPIDOPTERA: PYRALIDAE). Liu, Su,Zhang, Yan-Ru,Zhou, Wen-Wu,Liang, Qing-Mei,Yuan, Xin,Cheng, Jiaan,Zhu, Zeng-Rong,Gong, Zhong-Jun,Gong, Zhong-Jun. 2013

[18]A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action. Zhang, Linbao,Yang, Dinglong,Wang, Qing,Wu, Huifeng,Pei, Dong,Cong, Ming,Li, Fei,Ji, Chenglong,Zhao, Jianmin,Zhang, Linbao,Yang, Dinglong,Wang, Qing,Wu, Huifeng,Pei, Dong,Cong, Ming,Li, Fei,Ji, Chenglong,Zhao, Jianmin,Zhang, Linbao,Yang, Dinglong,Yuan, Zeyi.

[19]Functional analysis and induction of four novel goose (Anser cygnoides) avian beta-defensins in response to salmonella enteritidis infection. Ma, Deying,Zhou, Caiyuan,Zhang, Mingyue,Zhou, Caiyuan,Zhang, Mingyue,Han, Zongxi,Shao, Yuhao,Liu, Shengwang.

[20]Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense. Xiu, Yunji,Hou, Libo,Wang, Yinghui,Gu, Wei,Meng, Qingguo,Wang, Wen,Xiu, Yunji,Hou, Libo,Wang, Yinghui,Gu, Wei,Meng, Qingguo,Wang, Wen,Xiu, Yunji,Liu, Xiaoqian.

作者其他论文 更多>>

微信小程序