Genome-wide identification of PP2A gene family in Camellia sinensis reveals the potential role of CsPP2A-TON2/FASS1 in abiotic stress

文献类型： 外文期刊

第一作者： Bhattacharjee, Surjit

作者： Bhattacharjee, Surjit;Mishra, Neelam;Miao, Ye;Lu, Honglin;Shen, Guoxin

作者机构：

关键词： Protein phosphatases; Genome-wide search; qRT-PCR; Stress-responsiveness; C. sinensis.; Drought stress; Salt stress; PP2A gene family

期刊名称：PEERJ （ 影响因子：2.4； 五年影响因子：2.7 ）

ISSN： 2167-8359

年卷期： 2025 年 13 卷

页码：

收录情况： SCI

摘要： Background Protein phosphatases (PP) play a crucial role in regulation of physiological response to various environmental stimuli in plants. Protein phosphatase 2A (PP2A) are a class of Ser/Thr protein phosphatases which are present widely across plant species and facilitate plant responses to biotic and abiotic stresses, and hormone treatment. Methods Using BLASTp, tea PP2A genes were found and their physicochemical characteristics (ExPASy), subcellular localization, and intron-exon structure (GSDS) were described. MEGA software was used to examine phylogenetic relationships, SMART online tool to analyze conserved domains, and PlantCARE tool to analyze cis-elements. The Heatmapper online tool was used to visualize expression profiles across plant tissues and stress conditions. Under controlled conditions, 2-year-old Camellia sinensis plants were exposed to methyl jasmonate (MeJA) stress treatments, cold drought, and salt stresses and qRT-PCR (2(-Delta Delta Ct) technique, CsACTIN as internal control) was used to validate the results. Results We found 11 PP2A genes in C. sinensis (CsPP2A) through a genome-wide search wherein Arabidopsis thaliana was taken as the reference genome. Further, a systematic study was conducted wherein physicochemical properties, phylogeny, gene structure and gene expression were explored. Analysis of cis-elements, gene ontology, and expression patterns of CsPP2A genes under various stresses, combined with prior research on the PP2A gene family in other plants, suggests that the PP2A family plays a role in regulating phytohormone (auxin, salicylic acid, gibberellin, abscisic acid, MeJA) responses during stress in C. sinensis. The real time PCR also confirmed the potential role of (TEA018948.1) CsPP2AB9/CsPP2A-TON2/FASS1 gene in abiotic stress responses. Conclusion This study offers potential goals for further in-depth investigation and functional analysis, alongside enhancing our understanding of the regulatory network of PP2A genes in C. sinensis.

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