Visualizing plant intracellular inorganic orthophosphate distribution

文献类型： 外文期刊

第一作者： Guo, Meina

作者： Guo, Meina;Ruan, Wenyuan;Li, Ruili;Xu, Lei;Zhang, Qianqian;Ren, Jianhao;Yi, Keke;Guo, Meina;Hani, Sahar;David, Pascale;Nussaume, Laurent;Zheng, Bingsong

作者机构：

期刊名称：NATURE PLANTS （ 影响因子：18.0； 五年影响因子：18.6 ）

ISSN： 2055-026X

年卷期： 2024 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Intracellular inorganic orthophosphate (Pi) distribution and homeostasis profoundly affect plant growth and development. However, its distribution patterns remain elusive owing to the lack of efficient cellular Pi imaging methods. Here we develop a rapid colorimetric Pi imaging method, inorganic orthophosphate staining assay (IOSA), that can semi-quantitatively image intracellular Pi with high resolution. We used IOSA to reveal the alteration of cellular Pi distribution caused by Pi starvation or mutations that alter Pi homeostasis in two model plants, rice and Arabidopsis, and found that xylem parenchyma cells and basal node sieve tube element cells play a critical role in Pi homeostasis in rice. We also used IOSA to screen for mutants altered in cellular Pi homeostasis. From this, we have identified a novel cellular Pi distribution regulator, HPA1/PHO1;1, specifically expressed in the companion and xylem parenchyma cells regulating phloem Pi translocation from the leaf tip to the leaf base in rice. Taken together, IOSA provides a powerful method for visualizing cellular Pi distribution and facilitates the analysis of Pi signalling and homeostasis from the level of the cell to the whole plant. Visualizing cellular Pi distribution is crucial for the understanding of Pi signalling and homeostasis in plants. Here Guo et al. developed a rapid colorimetric Pi imaging method to reveal the intracellular Pi distribution and related regulators.

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