Quantitative PCR detection for abalone shriveling syndrome-associated virus

文献类型： 外文期刊

第一作者： Jiang Jing-Zhe

作者： Jiang Jing-Zhe;Zhang Han;Guo Zhi-Xun;Liu Guang-Feng;Su You-Lu;Wang Jiang-Yong;Zhu Zhen-Ni;Liang Ya-Yu;Zhang Han

作者机构：

关键词： Abalone;Haliotis diversicolor;Abalone shriveling syndrome-associated virus;Quantitative real time PCR;SYBR Green

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2012 年 184 卷 1-2 期

页码：

收录情况： SCI

摘要： Haliotis diversicolor (small abalone) is an important seafood found along the southern coast of China. Since 1999, the yields of cultured abalone in China have been severely affected by an epidemic of continuous outbreaks of a fatal disease. A novel double-stranded DNA virus, abalone shriveling syndrome-associated virus (AbSV), was proven to be one of the main causative agent. Although the pathogenicity and genome of AbSV has been ascertained, the epidemiology of AbSV remains to be investigated. In this study, four pairs of AbSV-specific primers were designed on the basis of the AbSV genome, and were tested for their specificities and sensitivities in quantitative real-time PCRs (qPCRs) after optimization of the annealing temperature. The 3F3/383 primer pair was finally chosen with a good specificity and high efficiency of amplification, with a detection limit of about 10 copies of recombinant plasmid containing AbSV genes in a 20-mu L reaction mixture. In the detection of AbSV in abalone samples along the southern coast of China, most of the diseased samples had more than 80 virus copies in 1 ng host genome DNA. AbSV was also demonstrated in mature hybrid (LY) and juvenile (JH) abalones from assays of healthy animals collected in recent years. (C) 2012 Elsevier B.V. All rights reserved.

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