Integrative ATAC-seq and RNA-seq analysis of myogenic differentiation of ovine skeletal muscle satellite cell

文献类型： 外文期刊

第一作者： Su, Yingxiao

作者： Su, Yingxiao;He, Siqi;Huang, Chang;Zhao, Qian;Pu, Yabin;He, Xiaohong;Jiang, Lin;Ma, Yuehui;Zhao, Qianjun;He, Siqi;Chen, Qian;Zhang, Hechun;Huang, Chang;Zhao, Qian

作者机构：

关键词： ATAC-seq; RNA-seq; Skeletal muscle satellite cell; Myogenic differentiation

期刊名称：GENOMICS （ 影响因子：4.4； 五年影响因子：4.2 ）

ISSN： 0888-7543

年卷期： 2024 年 116 卷 3 期

页码：

收录情况： SCI

摘要： Skeletal muscle satellite cells (SMSCs) play an important role in regulating muscle growth and regeneration. Chromatin accessibility allows physical interactions that synergistically regulate gene expression through enhancers, promoters, insulators, and chromatin binding factors. However, the chromatin accessibility altas and its regulatory role in ovine myoblast differentiation is still unclear. Therefore, ATAC-seq and RNA-seq analysis were performed on ovine SMSCs at the proliferation stage (SCG) and differentiation stage (SCD). 17,460 DARs (differential accessibility regions) and 3732 DEGs (differentially expressed genes) were identified. Based on joint analysis of ATAC-seq and RNA-seq, we revealed that PI3K-Akt, TGF- beta and other signaling pathways regulated SMSCs differentiation. We identified two novel candidate genes, FZD5 and MAP2K6 , which may affect the proliferation and differentiation of SMSCs. Our data identify potential cis regulatory elements of ovine SMSCs. This study can provide a reference for exploring the mechanisms of the differentiation and regeneration of SMSCs in the future.

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