Replicase components and the untranslated region of RNA2 synergistically regulate pathogenicity differentiation among different isolates of cucumber mosaic virus

文献类型： 外文期刊

第一作者： Wang, Yalan

作者： Wang, Yalan;Liu, Shanshan;Wang, Zhao;Hao, Kaiqiang;Wu, Yueming;Yu, Chengming;Yuan, Xuefeng;Liu, Chunju;Liu, Shanshan

作者机构：

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 294 卷

页码：

收录情况： SCI

摘要： Changes in critical sites of virus-encoded protein or cis-acting element generally determine pathogenicity differentiation among different isolates of the same plant virus. Cucumber mosaic virus (CMV) isolates, which exhibit the most extensively known host range, demonstrate notable pathogenicity differentiation. This study focuses on the severe isolate CMVFny and mild isolate CMV TA-pe , both affecting several species within the Solanaceae family, to identify the key factors regulating pathogenicity differentiation. Through a pseudo-recombination assay, the principal RNA segments regulating the pathogenicity of two CMV isolates were localized to RNA1 and RNA2, with a particular emphasis on RNA2. By generating chimeric mutants on RNA1 or RNA2 of the two isolates, the pathogenicity differentiation was suggested to be mainly associated with protein 1a of RNA1 as well as the synergistic interactions involving protein 2a as well as the 5 '-untranslated region (UTR) and the 3 '-UTR of RNA2. Moreover, the influence of protein 1a of RNA1 and protein 2a, 5 '-UTR, and 3 '-UTR of RNA2 on pathogenicity differentiation exhibited a coevolutionary pattern. This coevolutionary pattern of proteins 1a and 2a, along with the UTRs of RNA2, was also corroborated in three additional CMV isolates: CMVZMBJ, CMV WF-Ch , and CMV JN-Cu . In addition, multiple phylogenetic tree analyses revealed a synchronous evolutionary pattern among proteins 1a and 2a and the 5 '-UTR and 3 '-UTR of RNA2. This study provided new insights into the pathogenicity differentiation of RNA viruses.

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