Purification and characteristics of serine protease from the head of pacific white shrimp

文献类型： 外文期刊

第一作者： Wu, Yanyan

作者： Wu, Yanyan;Li, Laihao;Yang, Xianqing;Diao, Shiqiang;Wang, Ping

作者机构：

关键词： Pacific white shrimp;serine protease;purification;characteristics;enzyme activity

期刊名称：FOOD SCIENCE AND BIOTECHNOLOGY （ 影响因子：2.391； 五年影响因子：2.108 ）

ISSN： 1226-7708

年卷期： 2012 年 21 卷 4 期

页码：

收录情况： SCI

摘要： Serine protease from the head of Pacific white shrimp was purified by the following techniques: ammonium sulfate fractionation, Q-Sepharose HP ion exchange chromatography, and Sephadex G-100 gel filtration. The molecular weight was estimated as 32.8 kDa using SDSPAGE. The optimum pH and temperature of the enzyme for the hydrolysis of casein were determined to be 10.0 and 40A degrees C. It was stable at pH range from 8.0 to 11.0 and had good thermal stability. Pb2+, Ca2+, Mg2+, Cu2+, and Mn2+ could active the enzyme certainly when Zn2+ and Hg2+ strongly inhibited the activity. The enzyme was inhibited by the general serine protease inhibitor (PMSF) and the specific trypsin inhibitors (TLCK, SBTI). The modification of various amino acid modifiers for the purified enzyme determined that the enzyme active center included tryptophan, histidine, and serine, moreover, arginine had a certain relationship with the enzyme activity.

分类号：

  • 相关文献

[1]Purification and characterization of chitinase from Gliocladium catenulatum strain HL-1-1. Wu, Sheng-Jun,Gao, Hui-Lan,Zhang, Yong-Hua,Li, Shi-Dong,Xie, Bing-Yan. 2012

[2]Soluble expression and enzymatic activity evaluation of protease from reticuloendotheliosis virus. Hu, Feng,Zhao, Yan,Qi, Xiaole,Cui, Hongyu,Gao, Yulong,Gao, Honglei,Liu, Changjun,Wang, Yongqiang,Zhang, Yanping,Li, Kai,Wang, Xiaomei,Wang, Yunfeng,Wang, Xiaomei,Wang, Yunfeng.

[3]Effect of cinnamaldehyde on melanosis and spoilage of Pacific white shrimp (Litopenaeus vannamei) during storage. Mu, Honglei,Chen, Hangjun,Fang, Xiangjun,Mao, Jinlin,Gao, Haiyan. 2012

[4]A novel serine protease with clip domain from scallop Chlamys farreri. Zhu, Ling,Song, Linsheng,Zhao, Jiangmin,Li, Chenghua,Xu, Wei,Zhu, Ling,Mao, Yuze. 2008

[5]Serine Proteases of Parasitic Helminths. Yang, Yong,Wen, Yun jun,Cheng, Shi Peng,Yang, Yong,Vallee, Isabelle,Boireau, Pascal,Cai, Ya Nan,Liu, Ming Yuan,Liu, Ming Yuan. 2015

[6]Purification, characterization. and gene cloning of an alkaline serine protease from a highly virulent strain of the nematode-endoparasitic fungus Hirsutella rhossiliensis. Liu, Xingzhong,Wang, Bin,Liu, Xiaoying,Wu, Wenping,Li, Shidong. 2009

[7]Pharmacokinetics of sulfamethoxazole and trimethoprim in Pacific white shrimp, Litopenaeus vannamei, after oral administration of single-dose and multiple-dose. Ma, Rongrong,Wang, Yuan,Zou, Xiong,Sun, Beibei,Fang, Wenhong,Fu, Guihong,Ma, Rongrong,Hu, Kun,Yang, Xianle.

[8]A NEW COMPLEX BIOFLOCCULANT PRODUCED BY MIX-STRAINS IN ALCOHOLIC WASTEWATER AND THE CHARACTERISTICS OF THE BIOFLOCCULANT. Wang, Xiangdong,Wang, Yina,Zhang, Wang,Chen, Jiao,Zhang, Fan,Xiao, Jun,Huang, Juan. 2011

[9]Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans. Cheng, Jianhui,Xu, Xin,Wang, Liangyan,Tian, Bing,Hua, Yuejin,Wang, Hu. 2015

[10]Characteristics of Antisense Transcript Promoters and the Regulation of Their Activity. Lin, Shudai,Zhang, Li,Luo, Wen,Zhang, Xiquan,Lin, Shudai,Zhang, Li,Luo, Wen,Zhang, Xiquan,Lin, Shudai,Zhang, Li,Luo, Wen,Zhang, Xiquan,Zhang, Li. 2016

[11]Characteristics of spermatogonial stem cells derived from neonatal porcine testis. Shi, R.,Bai, Y.,Wei, H.,Zhang, X.,Li, L.,Jiang, Q.,Wang, C.,Qin, L.,Zhang, S.,Shi, R.,Li, S.,Cai, J.,Zhang, X.,Tian, X. C.. 2015

[12]Construction of cDNA Library and Analysis of SSRs in ESTs of Litchi (Litchi chinensis Sonn.). Xiang, X.,Sun, Q. M.,Ou, L. X.,Zhao, J. S.,Chen, J. Z.,Cai, C. H.. 2012

[13]Zea mays NAC transcription factor family members: their genomic characteristics and relationship with drought stress. Li, Liang,Ma, Yiwen,Li, Liang,Ma, Yiwen,Zhang, Shihuang,Hao, Zhuanfang,Li, Xinhai. 2015

[14]Effect of heat treatment to sweet potato flour on dough properties and characteristics of sweet potato-wheat bread. Carballo Perez, Isela,Mu, Tai-Hua,Zhang, Miao,Ji, Lei-Lei,Carballo Perez, Isela,Mu, Tai-Hua,Zhang, Miao,Ji, Lei-Lei,Carballo Perez, Isela.

[15]Attempt at conserving the genetic resources of Hu sheep by fibroblast line cryopreservation. Li, Xiangchen,Hu, Pengfei,He, Xiaohong,Lu, Taofeng,Guan, Weijun,Ma, Yuehui.

[16]Agricultural vulnerability and adaptation in developing countries: the Asia-Pacific region. Luo, QY,Lin, E.

[17]Structure, expression profile, and evolution of the sucrose synthase gene family in peach (Prunus persica). Zhang, Chunhua,Yu, Mingliang,Ma, Ruijuan,Shen, Zhijun,Zhang, Binbin,Korir, Nicholas Kibet.

[18]First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha. Ma, Duanzheng,Wang, Hexiang,Sekete, Malota,Ma, Duanzheng,Wang, Hexiang,Wang, Bo,Ng, Tzibun. 2012

[19]Purification and characterization of a cis-epoxysuccinic acid hydrolase from Bordetella sp strain 1-3. Li, Xia,Lu, Hongbo,Ma, Xiaohang,Kai, Lei,Guo, Kangping,Zhao, Yuhua,Li, Xia,Xu, Tongcheng. 2010

[20]An acid-tolerant lectin coupled with high Hg2+ potentiated hemagglutination enhancing property purified from Amanita hemibapha var. ochracea. Ma, Duanzheng,Wang, He Xiang,Sekete, Malota,Ma, Duanzheng,Wang, He Xiang,Sekete, Malota,Wang, Bo,Gong, Zhiyuan,Ng, Tzi Bun. 2014

作者其他论文 更多>>

微信小程序