Characterization of skin ulceration syndrome associated microRNAs in sea cucumber Apostichopus japonicus by deep sequencing

文献类型： 外文期刊

第一作者： Li, Chenghua

作者： Li, Chenghua;Feng, Weida;Xia, Changge;Su, Xiurong;Jin, Chunhua;Zhou, Tingting;Zeng, Yuan;Li, Taiwu;Qiu, Lihua;Li, Taiwu

作者机构：

关键词： Apostichopus japonicus;Skin ulceration syndrome;MicroRNA;Deep sequencing

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2012 年 33 卷 2 期

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) constitute a family of small RNA species which have been demonstrated to be one of key effectors in mediating host-pathogen interaction. In this study, two haemocytes miRNA libraries were constructed with deep sequenced by illumina Hiseq2000 from healthy (L1) and skin ulceration syndrome Apostichopus japonicus (L2). The high throughput solexa sequencing resulted in 9,579,038 and 7,742,558 clean data from L1 and L2, respectively. Sequences analysis revealed that 40 conserved miRNAs were found in both libraries, in which let-7 and mir-125 were speculated to be clustered together and expressed accordingly. Eighty-six miRNA candidates were also identified by reference genome search and stem-loop structure prediction. Importantly, mir-31 and mir-2008 displayed significant differential expression between the two libraries according to FPKM model, which might be considered as promising targets for elucidating the intrinsic mechanism of skin ulceration syndrome outbreak in the species. (c) 2012 Elsevier Ltd. All rights reserved.

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