Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho

文献类型： 外文期刊

第一作者： Liu, Shufang

作者： Liu, Shufang;Liu, Hongbo;Yuan, Yanjiao;Dai, Fangqun;Zhuang, Zhimeng;Liu, Hongbo;Lin, Lin;Yuan, Yanjiao

作者机构：

关键词： Solenocera crassicornis;Solenocera melantho;microsatellite marker;cross-species amplification

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2012 年 13 卷 7 期

页码：

收录情况： SCI

摘要： Twenty microsatellite markers were isolated and characterized for Solenocera crassicornis from a (GT)(13)-enriched genomic library. Their polymorphisms were investigated using 44 wild individuals from the South Yellow Sea. Our investigation revealed that all the markers were polymorphic. The number of alleles per locus varied from 6 to 19 with an average of 12.35. The observed and expected heterozygosities ranged from 0.400 to 0.977 and from 0.609 to 0.940, with averages of 0.788 and 0.859, respectively. Four loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni's correction. Cross-species amplification was also conducted in Solenocera melantho collected from the East China Sea. The result showed that 14 loci could be amplified from Solenocera melantho DNAs. These polymorphic markers would be useful for assessment of genetic variation and population structure of S. crassicornis and S. melantho.

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