Heterologous expression of taxane genes confers resistance to fall armyworm in Nicotiana benthamiana

文献类型： 外文期刊

第一作者： Xu, Zhenlu

作者： Xu, Zhenlu;Zhai, Yaohua;Yan, Da;Ren, Guangming;Zhang, Lijun;Yuan, Ye;Li, Fuguang;Ren, Maozhi;Mo, Huijuan;Chang, Huimin;Li, Fuguang;Ren, Maozhi;Mo, Huijuan;Ge, Pengliang;Ren, Maozhi;Wang, Ruoyan;Li, Wentao

作者机构：

关键词： Fall armyworm; Paclitaxel; Ectopic expression; Sf9 cell line

期刊名称：PLANT CELL REPORTS （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0721-7714

年卷期： 2024 年 43 卷 4 期

页码：

收录情况： SCI

摘要： Fall armyworm (FAW) is a serious agricultural pest. Genetic engineering techniques have been used to create pest-resistant plant varieties for reducing pest damage. Paclitaxel is a diterpenoid natural metabolite with antineoplastic effects in medicine. However, the effects of taxanes on the growth and development of lepidoptera pests, such as the FAW, are unknown. Here, selected paclitaxel precursor biosynthesis pathway genes, taxadiene synthase, taxane 5 alpha-hydroxylase, and taxane 13 alpha-hydroxylase, were engineered in the heterologous host Nicotiana benthamiana plants. Bioassay experiments showed that the transgenic N. benthamiana plants displayed improved resistance to FAW infestation, with degeneration of gut tissues and induced expression of apoptosis-related genes. Cytotoxicity experiment showed that the paclitaxel precursor, 10-deacetylbaccatin III, is cytotoxic to Sf9 cells, causing cell cycle arrest at the G2/M phase and disorder of the cytoskeleton. Metabolome analysis showed that heterologous expression of taxane genes in N. benthamiana affected the digestive system, steroid hormone and purine metabolism pathways of FAW larvae. In summary, this study provides a candidate approach for FAW control.

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