Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite

文献类型： 外文期刊

第一作者： Liang, Huan

作者： Liang, Huan;Ning, Guobao;Lu, Xiaokang;Li, Canpeng;Liu, Hongcheng;Lin, Haojian;Liu, Fei;Ma, Siying;Zhao, Hui

作者机构：

关键词： Staphylococcus aureus enterotoxin; Electrochemical immunosensor; Colorimetric assay; MOF@borophene composite; Dual-functional Fe-N-C signal atom catalyst

期刊名称：FOOD SCIENCE AND HUMAN WELLNESS （ 影响因子：7.0； 五年影响因子：8.3 ）

ISSN：

年卷期： 2024 年 13 卷 4 期

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收录情况： SCI

摘要： Sensitive detection of Staphylococcus aureus enterotoxin B (SEB) is of importance for preventing food poisoning from threatening human health. In this work, an electrochemical and colorimetric dual-signal detection assay of SEB was developed. The probe (Ab2/AuPt@Fe-N-C) was bound to SEB captured by Ab1, where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal. Furthermore, the probe catalyzed the oxidation of 3,3',5,5'-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm. Once the target was captured and formed a sandwich-like complex, the color changed from colorless to blue. SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.000 2-10.000 0 and 0.000 5-10.000 0 ng/mL, with limits of detection of 0.066 7 and 0.167 0 pg/mL, respectively. The dual-signal biosensor was successfully used to detect SEB in milk and water samples, which has great potential in toxin detection in food and the environment. (c) 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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