文献类型： 外文期刊
第一作者： Song, Jingze
作者： Song, Jingze;Li, Na;Dong, Xinze;Xie, Jianping;Lai, Hongqiao;Zhu, Hengzhi;He, Kongwang;Wen, Libin;Xiao, Qi;Xie, Jianping;He, Kongwang;Wen, Libin;Xiao, Qi;Xie, Jianping;He, Kongwang;Wen, Libin;Xiao, Qi;Xie, Jianping;He, Kongwang;Wen, Libin;Xiao, Qi;Suolang, Sizhu
作者机构：
关键词： RalGDS; isoform; LLC-PK1 cells; frameshift mutation; molecular evolution
期刊名称：CURRENT ISSUES IN MOLECULAR BIOLOGY （ 影响因子：3.0； 五年影响因子：3.2 ）
ISSN： 1467-3037
年卷期： 2025 年 47 卷 7 期
页码：
收录情况： SCI
摘要： This study investigated the isoforms of porcine-origin Ral guanine nucleotide dissociation stimulator (RalGDS) in LLC-PK1 cells using reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. Through segmented amplification, sequence assembly, and comparative genomics analysis, seven RalGDS isoforms were identified, characterized by insertions, deletions, and frameshift mutations. These genetic variations may significantly alter RalGDS's protein structure and function, potentially impacting its role in Ral GTPase-mediated signaling pathways. This work provides foundational insights into the genetic diversity of porcine RalGDS and its implications for porcine physiology and economically significant traits.
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