Detection and molecular characterisation of intestinal parasites in the South China tiger Panthera tigris amoyensis (Hilzheimer)

文献类型: 外文期刊

第一作者: Chiu, Hung-Chuan

作者: Chiu, Hung-Chuan;Fan, Kewei;Sun, Xiaoshuang;Huang, Cuiqin;Chiu, Hung-Chuan;Fan, Kewei;Huang, Cuiqin;Sun, Xiaoshuang;Lin, Kaixiong;Chen, Tengteng;Yang, Fei;Qiu, Yunfei;Wei, Dongxia

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关键词: Mini-FLOTAC; Toxascaris leonina; Cystoisospora sp; Felidae; Meihua; Mountain; China

期刊名称:FOLIA PARASITOLOGICA ( 影响因子:1.614; 五年影响因子:1.907 )

ISSN: 0015-5683

年卷期: 2021 年 68 卷

页码:

收录情况: SCI

摘要: Parasitic infections of the South China tigers in the Meihua Mountains have not been explored previously. Faeces of 22 South China tigers from the China Tiger Park in the Meihua Mountains were examined. Eggs of ascaridoid nematodes and oocysts of coccidia were detected by Mini-FLOTAC assay. Morphological observation and molecular characterisation of the oocysts were carried out. The prevalence of Toxascaris leonina (von Linstow, 1902) was 18% (4/22), and the highest egg per gram (EPG) count in the faeces was 27,150. The prevalence of Cystoisospora sp. was 45% (1 0/22) and the highest oocysts per gram (OPG) in the faeces was 6,000. In addition, we found one ascaridoid nematode in the South China tiger's faeces and was molecularly and morphologically identified as T. leonina. The oocysts in the faeces were sporulated in vitro and identified as Cystoisospora sp. Amplification of full-length internal transcribed spacers (ITS) resulted in sequences 1,622 bp long. Using the sequences, Cystoisospora sp. of the South China tiger was closest to Isospora belli (Wenyon, 1923) and Cystoisospora suis (Biester, 1934).

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