Vaccination with a DNase II recombinant protein against Trichinella spiralis infection in pigs

文献类型： 外文期刊

第一作者： Xu, Daoxiu

作者： Xu, Daoxiu;Tang, Bin;Yang, Yong;Liu, Mingyuan;Liu, Xiaolei;Cai, Xuepeng;Jia, Wanzhong;Luo, Xuenong;Yan, Hongbin;Zhang, Zhuangzhi;Lin, Jiaojiao

作者机构：

关键词： Trichinella spiralis; Pig; DNaseII-7; Vaccine; Protective effect

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

ISSN： 0304-4017

年卷期： 2021 年 297 卷

页码：

收录情况： SCI

摘要： Trichinellosis caused by Trichinella spiralis (T. spiralis) is an important public health problem. DNase II is an acidic endonuclease that catalyzes the degradation of DNA into oligonucleotides. DNase II-7 has been detected at the adult stage of T. spiralis and has been examined in excretory/secretory products. Previous studies have indicated that the DNase II-7 recombinant protein has a high rate of protection against T. spiralis infection in mice. In the present study, the protective effect of DNase II-7 recombinant protein against T. spiralis infection in Large White pigs was further explored. The humoral and cellular immune responses to the DNase II-7 recombinant protein were evaluated, including the dynamic trends of specific IgG, IgG1, IgG2a and IgM antibodies levels, as well as the levels of Th1 (IFN-gamma and IL-2) and Th2 (IL-10 and IL-4) cytokines in serum. Our results showed that a Th1 dominated Th1/Th2 mixed immune response was induced by the DNase II-7 recombinant protein for all the time or a short period after vaccination. And the DNase II-7 recombinant protein induced partial protection against T. spiralis infection in pigs, compared to the control group. Our results showed that the DNase II-7 recombinant protein group displayed a 45.7 % reduction in the muscle larvae burden five weeks after being challenged. This study suggested that DNaseII-7 recombinant protein could be used as a potential candidate vaccine against T. spiralis infection in pigs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序