Dual role of IGF-II in oocyte maturation in southern flounder Paralichthys lethostigma: Up-regulation of mPR alpha and resumption of meiosis

文献类型： 外文期刊

第一作者： Picha, Matthew E.

作者： Picha, Matthew E.;Shi, Bao;Thomas, Peter;Shi, Bao;Shi, Bao

作者机构：

关键词： Membrane progestin receptor;Insulin-like growth factor;Oocyte maturation;Southern flounder

期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）

ISSN： 0016-6480

年卷期： 2012 年 177 卷 2 期

页码：

收录情况： SCI

摘要： Increasing evidence suggests a regulatory role for the IGF system in teleost oocyte maturation (OM). Our objectives were to determine if IGF-I and IGF-II regulate different stages of OM in southern flounder (Paralichthys lethostigma) and to identify the likely maturation-inducing steroid (MIS) in this species. The most abundant final product of ovarian steroidogenesis assays eluted at the position of 17,20 beta,21-trihydroxy-4-pregnen-3-one (20 beta-S). 20 beta-S was also more potent in inducing germinal vesicle breakdown (GVBD) of maturationally-competent oocytes than other teleost MISs. IGF-II (100 nM) induced maturational competence (OMC), as greater GVBD was induced after incubation with IGF-II + 20 beta-S compared to that of the 20 beta-S + 20 beta-S or IGF-II + no treatment group. Incubation with IGF-II (100 nM) for 4-8 h significantly increased ovarian membrane progestin receptor alpha (mPR alpha or Paqr7b) mRNA levels 12-15% and mPR alpha protein levels 75-101%. Further, the IGF-II-induced increase in mPR alpha protein concentrations was partially blocked by pretreatment with Wortmannin, a Pik3 inhibitor, and PD 098,059, a Mapk inhibitor. Both IGF-I and -II (100 nM) induced GVBD of maturationally-competent oocytes was blocked by incubation with cycloheximide. Incubation with D,L-Aminoglutethimide decreased IGF-II-induced GVBD but had no effect on IGF-I-induced GVBD. IGF-I and -II were also able to induce GVBD of maturationally-incompetent oocytes, and elicited 75% and 135% greater GVBD, respectively, than hCG + 20 beta-S at 100 nM. In conclusion, we show that 20 beta-S is the likely MIS in this species and that IGF-I and -II are also able to induce GVBD. Further, IGF-II not only induces OMC but also up-regulates ovarian mPR alpha mRNA and protein through Pik3- and Mapk-dependent pathways. This is the first demonstration of mPR alpha regulation by an IGF in any vertebrate species. (c) 2012 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Establishment, characterization and virus susceptibility of a kidney-derived cell line from southern flounder, Paralichthys lethostigma Jordan & Gilbert. Wang, N.,Wang, X-L,Sha, Z-X,Tian, Y-S,Chen, S-L,Chen, S-L. 2011

[2]Role of animal pole protuberance and microtubules during meiosis in sea cucumber Apostichopus japonicus oocytes. Chang Yaqing,Yu Jiaping,Sun Huiling,Pang Zhenguo,Pang Zhenguo. 2010

[3]IGF-I slightly improves nuclear maturation and cleavage rate of bovine oocytes exposed to acute heat shock in vitro. Qi Meiyu,Liu, Di.

[4]PPAR gamma is regulated by miR-27b-3p negatively and plays an important role in porcine oocyte maturation. Song, Chunlei,Liang, Xiaojuan,Tao, Cong,Li, Kui,Wang, Yanfang,Yao, Jing,Cao, Chunwei,Huang, Jiaojiao,Han, Ziqiang,Zhang, Yang,Qin, Guosong,Zhao, Jianguo,Yao, Jing,Cao, Chunwei,Huang, Jiaojiao,Zhang, Yang,Qin, Guosong,Zhao, Jianguo,Han, Ziqiang,Li, Chengbo,Yang, Haoran.

[5]Role of the insulin-like growth factor system in epiphyseal cartilage on the development of Langshan and Arbor Acres chickens, Gallus domesticus. Lu, F. Z.,Wang, X. X.,Luo, Y. H.,Liu, H. L.,Lu, F. Z.,Jiang, Z. Y.,Li, X. F.. 2010

[6]Expression of the insulin-like growth factor system in skeletal muscle during embryonic and postnatal development in the first filial generation pigs from Erhualian and Yorkshire reciprocal crosses. Wang, Lijuan,Lin, Fei,Jiang, Baochun,Dong, Fulu,Liu, Honglin,Zhang, Guangmin. 2011

[7]iTRAQ Protein Profile Differential Analysis of Dormant and Germinated Grassbur Twin Seeds Reveals that Ribosomal Synthesis and Carbohydrate Metabolism Promote Germination Possibly Through the PI3K Pathway. Zhang, Guo-Liang,Zhu, Yue,Fu, Wei-Dong,Zhang, Rui-Hai,Zhang, Yan-Lei,Song, Zhen,Zhu, Yue,Wang, Peng,Xia, Gui-Xian,Wu, Jia-He.

[8]Effects of nutrition level of concentrate-based diets on growth performance and carcass characteristics of Hainan black goats. Wang, Dingfa,Zhou, Luli,Zhou, Hanlin,Hou, Guanyu,Li, Mao,Shi, Liguang,Huang, Xianzhou,Guan, Song.

作者其他论文 更多>>

微信小程序