An alternative splicing variant of PtRD26 delays leaf senescence by regulating multiple NAC transcription factors in Populus

文献类型： 外文期刊

第一作者： Wang, Hou-Ling

作者： Wang, Hou-Ling;Zhang, Yi;Wang, Ting;Yang, Qi;Yang, Yanli;Yin, Weilun;Xia, Xinli;Guo, Hongwei;Li, Zhonghai;Wang, Ting;Yang, Qi;Yang, Yanli;Yin, Weilun;Xia, Xinli;Li, Ze;Li, Bosheng;Wen, Xing;Li, Wenyang;Guo, Hongwei

作者机构：

期刊名称：PLANT CELL （ 影响因子：11.277； 五年影响因子：12.061 ）

ISSN： 1040-4651

年卷期： 2021 年 33 卷 5 期

页码：

收录情况： SCI

摘要： During leaf senescence, the final stage of leaf development, nutrients are recycled from leaves to other organs, and therefore proper control of senescence is thus critical for plant fitness. Although substantial progress has been achieved in understanding leaf senescence in annual plants, the molecular factors that control leaf senescence in perennial woody plants are largely unknown. Using RNA sequencing, we obtained a high-resolution temporal profile of gene expression during autumn leaf senescence in poplar (Populus tomentosa). Identification of hub transcription factors (TFs) by co-expression network analysis of genes revealed that senescence-associated NAC family TFs (Sen-NAC TFs) regulate autumn leaf senescence. Age-dependent alternative splicing (AS) caused an intron retention (IR) event in the pre-mRNA encoding PtRD26, a NAC-TF. This produced a truncated protein PtRD26(IR), which functions as a dominant-negative regulator of senescence by interacting with multiple hub Sen-NAC TFs, thereby repressing their DNA-binding activities. Functional analysis of senescence-associated splicing factors identified two U2 auxiliary factors that are involved in AS of PtRD26(IR) . Correspondingly, silencing of these factors decreased PtRD26(IR) transcript abundance and induced early senescence. We propose that an age-dependent increase of IR splice variants derived from Sen-NAC TFs is a regulatory program to fine tune the molecular mechanisms that regulate leaf senescence in trees.

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