Nucleosome Assembly Protein 1, Nap1, Is Required for the Growth, Development, and Pathogenicity of Magnaporthe oryzae

文献类型： 外文期刊

第一作者： Wang, Qing

作者： Wang, Qing;Wang, Jing;Huang, Pengyun;Huang, Zhicheng;Li, Yan;Lu, Jianping;Liu, Xiaohong;Lin, Fucheng;Lin, Fucheng

作者机构：

关键词： rice blast; virulence; septin ring; cell wall integrity; histone; appressorium

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 14 期

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收录情况： SCI

摘要： Magnaporthe oryzae is the causal agent of rice blast, leading to significant reductions in rice and wheat productivity. Nap1 is a conserved protein in eukaryotes involved in diverse physiological processes, such as nucleosome assembly, histone shuttling between the nucleus and cytoplasm, transcriptional regulation, and the cell cycle. Here, we identified Nap1 and characterized its roles in fungal development and virulence in M. oryzae. MoNap1 is involved in aerial hyphal and conidiophore differentiation, sporulation, appressorium formation, plant penetration, and virulence. Delta Monap1 generated a small, elongated, and malformed appressorium with an abnormally organized septin ring on hydrophobic surfaces. Delta Monap1 was more sensitive to cell wall integrity stresses but more resistant to microtubule stresses. MoNap1 interacted with histones H(2)A and H2B and the B-type cyclin (Cyc1). Moreover, a nuclear export signal (NES) domain is necessary for Nap1's roles in the regulation of the growth and pathogenicity of M. oryzae. In summary, NAP1 is essential for the growth, appressorium formation, and pathogenicity of M. oryzae.

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