Salicylic acid fights against Fusarium wilt by inhibiting target of rapamycin signaling pathway in Fusarium oxysporum

文献类型： 外文期刊

第一作者： Li, Linxuan

作者： Li, Linxuan;Zhu, Tingting;Feng, Li;Ren, Maozhi;Li, Linxuan;Zhu, Tingting;Feng, Li;Ren, Maozhi;Li, Linxuan;Zhu, Tingting;Feng, Li;Ren, Maozhi;Song, Yun;Kear, Philip James;Riseh, Rooallah Saberi;Sitohy, Mahmoud;Datla, Raju

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关键词： Salicylic acid; Target of rapamycin; Hyphal growth; Pathogenicity; Fusarium oxysporum

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：12.822； 五年影响因子：11.664 ）

ISSN： 2090-1232

年卷期： 2022 年 39 卷

页码：

收录情况： SCI

摘要： Introduction: Biofungicides with low toxicity and high efficiency are a global priority for sustainable agricultural development. Phytohormone salicylic acid (SA) is an ancient medicine against various diseases in humans and activates the immune system in plants, but little is known of its function as a biofungicide. Objectives: Here, Fusarium oxysporum, the causal agent of devastating Fusarium wilt and immunodepressed patients, was used as a model system to explore whether SA can enter the pathogen cells and suppress key targets of the pathogen. Methods: Oxford Nanopore MinION sequencing and high-throughput chromosome conformation capture (Hi-C) sequencing were used to analyzed the genome of F. oxysporum. In addition, RNA-seq, qRT-PCR, and western blotting were conducted to detect gene and protein expression levels.Results: We isolated and sequenced the genome of F. oxysporum from potato dry rot, and the F. oxysporum included 12 chromosomes and 52.3 Mb genomic length. Pharmacological assays showed that exogenous application of SA can efficiently arrest hyphal growth, spore production, and pathogenicity of F. oxysporum, whereas endogenous salicylate hydroxylases significantly detoxify SA. The synergistic growth inhibition of F. oxysporum was observed when SA was combined with rapamycin. Kinase assays showed that SA inhibits FoTOR complex 1 (FoTORC1) by activating FoSNF1 in vivo. Transgenic potato plants with the interference of FoTOR1 and FoSAH1 genes inhibited the invasive growth of hyphae and significantly prevented the occurrence of Fusarium wilt. Conclusion: This study revealed the underlying mechanisms of SA against F. oxysporum and provided insights into SA in controlling various fungal diseases by targeting the SNF1-TORC1 pathway of pathogens. (c) 2022 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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