文献类型： 外文期刊
第一作者： Liu, Ye
作者： Liu, Ye;Bai, Yanju;Liu, Ye;Fan, Xinyue;Luan, Yameng;Zhao, Yu;Ji, Weiqin;Li, Naihui;Wu, Xiaoyun;Cheng, Xiaofei;Li, Yong
作者机构：
关键词： 8K; cysteine-rich protein; potato mop-top virus; viroporin
期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.6 ）
ISSN： 1464-6722
年卷期： 2025 年 26 卷 9 期
页码：
收录情况： SCI
摘要： Potato mop-top virus (PMTV) is the causal agent of potato tuber spraing disease, which causes significant economic losses to potato production worldwide. The 3 '-proximal end of PMTV genomic RNA3 encodes an 8 kDa cysteine-rich protein (8K) that is not essential for replication and movement but contributes to virus infection and symptom development. Here, we demonstrate that PMTV 8K forms endomembrane multimers, alters the membrane permeability of Escherichia coli, and possesses potassium and proton conductance activity. In addition, our data reveal that two conserved cysteine residues in the central hydrophobic alpha-helix are essential for the viroporin activity. These results not only deepen our understanding of the function of PMTV 8K but also provide new insights into the diversity and origin of plant viral viroporins.
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