An intronic SNP affects skeletal muscle development by regulating the expression of TP63

文献类型： 外文期刊

第一作者： Chen, Yufen

作者： Chen, Yufen;Wang, Zhen;Qu, Xiaolu;Song, Bangmin;Tang, Yueting;Yi, Guoqiang;Chen, Yufen;Li, Bugao;Cao, Guoqing;Yi, Guoqiang;Yi, Guoqiang

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关键词： TP63; myoblasts; proliferation; allelic variation; MEF2A

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：2.6； 五年影响因子：3.1 ）

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年卷期： 2024 年 11 卷

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收录情况： SCI

摘要： Background: Porcine skeletal muscle development is pivotal for improving meat production. TP63, a transcription factor, regulates vital cellular processes, yet its role in skeletal muscle proliferation is unclear. Methods: The effects of TP63 on skeletal muscle cell viability and proliferation were investigated using both mouse and porcine skeletal muscle myoblasts. Selective sweep analysis in Western pigs identified TP63 as a potential candidate gene for skeletal muscle development. The correlation between TP63 overexpression and cell proliferation was assessed using quantitative real-time PCR (RT-qPCR) and 5-ethynyl-2'-deoxyuridine (EDU). Results: The study revealed a positive correlation between TP63 overexpression and skeletal muscle cell proliferation. Bioinformatics analysis predicted an interaction between MEF2A, another transcription factor, and the mutation site of TP63. Experimental validation through dual-luciferase assays confirmed that a candidate enhancer SNP could influence MEF2A binding, subsequently regulating TP63 expression and promoting skeletal muscle cell proliferation. Conclusion: These findings offer experimental evidence for further exploration of skeletal muscle development mechanisms and the advancement of genetic breeding strategies aimed at improving meat production traits.

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