Combined virome analysis and metagenomic sequencing to reveal the viral communities and risk of virus-associated antibiotic resistance genes during composting

文献类型： 外文期刊

第一作者： Yue, Zhengfu

作者： Yue, Zhengfu;Liu, Beibei;Li, Qinfen;Zou, Yukun;Zhang, Jing;Zhang, Jing;Zhang, Jing;Wang, Xingxiang;Li, Lirong;Yu, Haiyang;Zhu, Dong;Yue, Zhengfu;Liu, Beibei;Li, Qinfen;Zou, Yukun;Zou, Yukun

作者机构：

关键词： Antibiotic resistance gene; Composting; Health risk; Non-thermophilic; Virus

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）

ISSN： 0304-3894

年卷期： 2023 年 459 卷

页码：

收录情况： SCI

摘要： The issue of antibiotic resistance genes (ARGs) pollution in manure has garnered significant attention, with viruses now being recognized as crucial carriers and disseminators of ARGs. However, the virus-associated ARG profiles and potential health risks in composts are still unclear. In this study, the viral communities and associated ARGs in biogas residue and pig faeces composts were profiled by virome analysis. The viral communities were dominated by Caudovirales, and non-thermophilic viruses were inactivated during composting. The diversity and abundance of ARGs were lower in virome than in metagenome, while ARGs' risk was greater in virome than in metagenome. There were six bacterial genera identified as viral hosts at the genomic level, Pseudomonas and Clostridium carried high-risk ARGs. Virus-associated ARGs in viral hosts had a higher risk rank than non-virus-associated ARGs. Composting reduced the diversity, abundance and risk of viral ARGs. The risk of ARGs in biogas residues was significantly lower than that of pig faeces in the initial period of composting, and the two different substracts equally less harmful after composting. These results revealed that viruses play a non negligible role in spreading ARGs, posing high risk to environmental and human health.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序