Study on Synthesis and Characterization of a Fluorescence Probe and Its Application for Determination of Superoxide Anion Radical

文献类型： 外文期刊

第一作者： Zhao, Yongqiang

作者： Zhao, Yongqiang;Lin, Hong;Zhao, Yongqiang;Li, Laihao;Yang, Xianqing;Hao, Shuxian;Cen, Jianwei;Wei, Ya;Qi, Bo;Huang, Hui

作者机构：

关键词： synthesis;characterization;fluorescence probe;superoxide anion radical

期刊名称：BIOTECHNOLOGY, CHEMICAL AND MATERIALS ENGINEERING, PTS 1-3

ISSN： 1022-6680

年卷期： 2012 年 393-395 卷

页码：

收录情况： SCI

摘要： A novel Schiff base, 2-(Salicylideneamino)pyridine (2-SAP) synthesized by 2-aminopyridine and salicylaldehyde was used as a fluorescence probe to determine superoxide anion radical (O-2(center dot-)) in this study. The synthetic product was characterized by IR, H-1-NMR and GC-MS methods. A fluorescence quenching reaction occurs between 2-SAP and O-2(center dot-), and the fluorescence intensity decreased significantly. The effect of pyrogallol concentration (2.0x10(-5) M to1.0x10(-3) M) was discussed in this study. In the range from 2.0x10(-5) M to 1.0x10(-4) M, relative fluorescence intensity (y) and pyrogallol concentration (x) shown a good linear relationship,.y=37.801x+76.854, r=0.993. Based on this phenomenon, the O-2(center dot-) generated by pyrogallol autoxidation could be determined simply with high sensitivity.

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