Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

文献类型： 外文期刊

第一作者： Chen, Ruipeng

作者： Chen, Ruipeng;Wang, Hui;Zhao, Yiguang;Nan, Xuemei;Du, Chunmei;Zhang, Fan;Luo, Qingyao;Yang, Liang;Xiong, Benhai;Wei, Wensong

作者机构：

关键词： cow mastitis; interleukin-6 (IL-6); immunofiltration assay (IFA); SERS nanotag

期刊名称：NANOMATERIALS （ 影响因子：5.719； 五年影响因子：5.81 ）

ISSN：

年卷期： 2022 年 12 卷 7 期

页码：

收录情况： SCI

摘要： Interleukin-6 (IL-6) is generally used as a biomarker for the evaluation of inflammatory infection in humans and animals. However, there is no approach for the on-site and rapid detection of IL-6 for the monitoring of mastitis in dairy farm scenarios. A rapid and highly sensitive surface enhanced Raman scattering (SERS) immunofiltration assay (IFA) for IL-6 detection was developed in the present study. In this assay, a high sensitivity gold core silver shell SERS nanotag with Raman molecule 4-mercaptobenzoic acid (4-MBA) embedded into the gap was fabricated for labelling. Through the immuno-specific combination of the antigen and antibody, antibody conjugated SERS nanotags were captured on the test zone, which facilitated the SERS measurement. The quantitation of IL-6 was performed by the readout Raman signal in the test region. The results showed that the detection limit (LOD) of IL-6 in milk was 0.35 pg mL(-1), which was far below the threshold value of 254.32 pg mL(-1). The recovery of the spiking experiment was 87.0-102.7%, with coefficients of variation below 9.0% demonstrating high assay accuracy and precision. We believe the immunosensor developed in the current study could be a promising tool for the rapid assessment of mastitis by detecting milk IL-6 in dairy cows. Moreover, this versatile immunosensor could also be applied for the detection of a wide range of analytes in dairy cow healthy monitoring.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序