文献类型： 外文期刊
第一作者： Fu, Xiumin
作者： Fu, Xiumin;Chen, Jiaming;Dai, Guangyi;Yang, Ziyin;Fu, Xiumin;Chen, Jiaming;Dai, Guangyi;Yang, Ziyin;Chen, Jiaming;Yang, Ziyin;Li, Jianlong;Tang, Jinchi;Li, Jianlong;Tang, Jinchi;Yang, Ziyin
作者机构：
关键词： Biosynthesis; Camellia sinensis; Carotenoids; Metabolic regulation; Tea; Shade; Chemical compounds; ?-Carotene (PubChem CID; 4369188); ?-Carotene (PubChem CID; 54603957); ?-Carotene (PubChem CID; 5281230)
期刊名称：FOOD CHEMISTRY-X （ 影响因子：6.443； 五年影响因子：6.443 ）
ISSN： 2590-1575
年卷期： 2022 年 14 卷
页码：
收录情况： SCI
摘要： Carotenoids contribute to tea leaf coloration and are the precursors of important aromatic compounds. Shading can promote the accumulation of carotenoids in tea leaves, but the underlying mechanism remains unknown. In the study, we analyzed the content and composition of carotenoids, and transcript levels and functions of related genes in carotenoid biosynthesis using HPLC, qRT-PCR, and heterologous expression system. It was found that long-term shading (14 days, 90% shading) significantly increased the total carotenoid content in tea leaves, and increased the expression of non-mevalonate pathway (MEP) genes (CsDXS1 and CsDXS3) and key genes in carotenoid synthesis pathway (CsPSY, CsLCYB, and CsLCYE). Long-term exposure to darkness (14 days, 0 lx) decreased the transcription of most carotenoid biosynthetic genes and adversely affected carotenoid accumulation. Furthermore, CsDXS1, CsDXS3, CsPSY, CsLCYB, and CsLCYE were functionally identified and contributed to the enhanced accumulation of carotenoids in tea leaves in response to long-term shading.
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