Comparison of white spot syndrome virus quantification of fleshy shrimp Fenneropenaeus chinensis in outdoor ponds between different growing seasons by TaqMan real-time polymerase chain reaction

文献类型： 外文期刊

第一作者： Meng, Xian-Hong

作者： Meng, Xian-Hong;Jang, In Kwon;Kim, Jong-Sheek;Kim, Bong-Rae;Meng, Xian-Hong

作者机构：

关键词： shrimp pond;Fenneropenaeus chinensis;TaqMan real-time PCR;white spot syndrome virus (WSSV)

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期： 2011 年 42 卷 12 期

页码：

收录情况： SCI

摘要： In the present study, we used TaqMan real-time polymerase chain reaction to quantify and compare infection of white spot syndrome virus (WSSV) with shrimp production of Fenneropenaeus chinensis cultured in outdoor ponds along the west coast of the South Korea. In 2007, a total of 60 specimens in summer and 116 specimens in autumn were collected from 12 growing-out ponds and 12 harvest ponds respectively. Pond harvest data were obtained from farmers. Of the summer samples, all specimens were WSSV positive, with a wide range of 12.47.0 x 107 (mean 7.5 x 106) copies ng-1 DNA; shrimp production was 1.7 metric tonnes per hectare (mt ha-1). Of the 116 autumn-sample specimens, 81 (69.8%) were WSSV positive; WSSV infection had been decreased dramatically, to 07.2 (mean 3.5) copies ng-1 DNA. Shrimp production of autumn ponds was 2.1 mt ha-1. Statistical analysis indicated that the difference in WSSV infections detected in summer and autumn was highly significant (P<0.01). In summer, seven ponds (58.3%) with low-WSSV infection loads (01000 WSSV copies ng-1 DNA) had shrimp production of 2.7 mt ha-1; the others had shrimp production of only 0.2 mt ha-1. The mean shrimp production between the two infection levels showed a highly statistically significant difference (P<0.01).

分类号：

  • 相关文献

[1]A TaqMan real-time PCR assay for survey of white spot syndrome virus (WSSV) infections in Litopenaeus vannamei postlarvae and shrimp of farms in different grow-out seasons. Meng, Xian-Hong,Jang, In Kwon,Seo, Hyung-Chul,Cho, Yeong-Rok,Meng, Xian-Hong.

[2]Identification, cloning and characterization of an extracellular signal-regulated kinase (ERK) from Chinese shrimp, Fenneropenaeus chinensis. Li, Xupeng,Kong, Jie,Li, Xupeng,Meng, Xianhong,Kong, Jie,Luo, Kun,Luan, Sheng,Cao, Baoxiang,Liu, Ning,Pang, Jinfei,Shi, Xiaoli. 2013

[3]The identification of microRNAs involved in the response of Chinese shrimp Fenneropenaeus chinensis to white spot syndrome virus infection. Li, Xupeng,Meng, Xianhong,Luo, Kun,Luan, Sheng,Shi, Xiaoli,Cao, Baoxiang,Kong, Jie,Luan, Sheng,Kong, Jie.

[4]Molecular cloning and characterization of a cathepsin B gene from the Chinese shrimp Fenneropenaeus chinensis. Li, Xupeng,Kong, Jie,Li, Xupeng,Meng, Xianhong,Kong, Jie,Luo, Kun,Luan, Sheng,Cao, Baoxiang,Liu, Ning,Pang, Jinfei,Shi, Xiaoli. 2013

[5]Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon1445 and Mon531. Yang, LT,Pan, AH,Zhang, KW,Yin, CS,Qian, BJ,Chen, JX,Huang, C,Zhang, DB. 2005

[6]Application and development of a TaqMan real-time PCR for detecting infectious spleen and kidney necrosis virus in Siniperca chuatsi. Lin, Qiang,Fu, Xiaozhe,Liu, Lihui,Liang, Hongru,Guo, Huizhi,Yin, Shuwen,Huang, Zhibin,Li, Ningqiu,Kumaresan, Venkatesh.

[7]Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR. Yang, LT,Ding, JY,Zhang, CM,Jia, JW,Weng, HB,Liu, WX,Zhang, DB. 2005

[8]A specific qualitative and real-time PCR detection of MON863 maize based on the 5 '-transgene integration sequence. Zhu, Hong,Jia, Junwei,Sun, Jianping,Zhao, Kai,Zhao, Xiao. 2008

[9]Pre-infestation of Tomato Plants by Aphids Modulates Transmission-Acquisition Relationship among Whiteflies, Tomato Yellow Leaf Curl Virus (TYLCV) and Plants. Ge, Feng,Tan, Xiao L.,Chen, Ju L.,Tan, Xiao L.,Liu, Tong X.,Tan, Xiao L.,Liu, Tong X.,Benelli, Giovanni,Desneux, Nicolas,Yang, Xue Q.. 2017

[10]Real-time PCR quantification of Bemisia tabaci (Homoptera : Aleyrodidae) B-biotype remains in predator guts. Zhang, Gui-Fen,Lue, Zhi-Chuang,Wan, Fang-Hao,Loevei, Gabor L.. 2007

[11]Molecular responses of calreticulin gene to Vibrio anguillarum and WSSV challenge in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun. 2014

[12]Isolation and characterization of homologous TRBP cDNA for RNA interference in Penaeus monodon. Yang, Lishi,Li, Xiaolan,Huang, Jianhua,Zhou, Falin,Su, Tianfeng,Jiang, Shigui,Li, Xiaolan. 2013

[13]The responsive expression of a chitinase gene in the ridgetail white prawn Exopalaemon carinicauda against Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun.

[14]Oral administration of Anabaena-expressed VP28 for both drug and food against white spot syndrome virus in shrimp. Jia, Xiao-Hui,Shi, Ding-Ji,Zhuang, Min-Min,Wang, Xiang,Jia, Rui,Zhang, Zheng-Yang,Sun, Yi-Hua,Qian, Wen-Yi,He, Pei-Min,Zhang, Chun-Li,Peng, Guo-Hong,Shi, Ding-Ji,Huang, Jie.

[15]The expression of prophenoloxidase mRNA in red swamp crayfish, Procambarus clarkii, when it was challenged. Yang, Keli,Li, Yanhe,Deng, Wei,Wang, Weimin,Li, Yanhe.

[16]The role of oncoprotein NM23 gene from Exopalaemon carinicauda is response to pathogens challenge and ammonia-N stress. Duan, Yafei,Zhang, Zhe,Li, Jitao,Li, Jian,Ge, Qianqian,Liu, Ping,Li, Jitao,Li, Jian,Ge, Qianqian,Liu, Ping.

[17]Genetic variation and evolutionary demography of Fenneropenaeus chinensis populations, as revealed by the analysis of mitochondrial control region sequences. Kong, Xiao Yu,Li, Yu Long,Li, Yu Long,Shi, Wei,Kong, Jie. 2010

[18]Construction of a genetic linkage map in Fenneropenaeus chinensis using SNP markers. Zhang, Jian-Yong,Wang, Wei-Ji,Kong, Jie,Wang, Qing-Yin. 2013

[19]Estimation of genetic parameters and breeding values in shrimp Fenneropenaeus chinensis using the REML/BLUP procedure. Zhang Tianshi,Kong Jie,Luan Sheng,Wang Qingyin,Luo Kun,Zhang Tianshi,Tian Yi. 2011

[20]Toxic dinoflagellate Alexandrium tamarense induces oxidative stress and apoptosis in hepatopancreas of shrimp (Fenneropenaeus chinensis). Liang Zhongxiu,Ren Hai,Liang Zhongxiu,Li Jian,Li Jitao,Tan Zhijun,Zhao Fazhen. 2014

作者其他论文 更多>>

微信小程序