Expression Profiles of Gonadotropins and Their Receptors during 17 alpha-Methyltestosterone Implantation-Induced Sex Change in the Orange-Spotted Grouper (Epinephelus coioides)

文献类型： 外文期刊

第一作者： Hu, Xuesong

作者： Hu, Xuesong;Liu, Xiaochun;Zhang, Yong;Li, Shuisheng;Sang, Qing;Wang, Qian;Luo, Wenna;Liu, Qizhi;Lu, Danqi;Meng, Zining;Lin, Haoran;Hu, Xuesong;Liu, Xiaochun;Zhang, Yong;Li, Shuisheng;Sang, Qing;Wang, Qian;Luo, Wenna;Liu, Qizhi;Lu, Danqi;Meng, Zining;Lin, Haoran;Hu, Xuesong;Zhang, Haifa;Lin, Haoran

作者机构：

期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.609； 五年影响因子：3.47 ）

ISSN： 1040-452X

年卷期： 2011 年 78 卷 6 期

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收录情况： SCI

摘要： It is known that the hypothalamic-pituitary-gonadal axis participates in the sex change of hermaphrodite teleosts, and gonadal steroid hormones mediate this physiological process. The secretion of gonadal steroids is directly regulated by signaling pathways involving gonadotropins (GtHs) and gonadotropin receptors (GtHRs) in teleosts. To gain insight into the involvement of GtH/GtHR systems in the sex change process, cDNAs encoding follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) were firstly isolated from gonads of orange-spotted grouper (Epinephelus coioides), a protogynous hermaphrodite fish. Reverse transcription-PCR (RT-PCR) analysis demonstrated that the expression of the FSHR was confined to the brain, pituitary gland, ovary, and testis, while the LHR was expressed only in the brain, ovary, and testis. Furthermore, the expression profiles of GtH subunits (FSH beta and LH beta) and their receptors were analyzed in parallel with the serum levels of estradiol-17 beta (E(2)), testosterone (T), and 11-ketotestosterone (11-KT) during 17 alpha-methyltestosterone (MT)-induced sex change. Quantitative real-time PCR determined that the abundances of FSH beta and FSHR were significantly inhibited after MT treatment for 2 and 4 weeks, but subsequently returned to the control level after 6 weeks. In contrast, the mRNA levels of LH beta and LHR were significantly elevated throughout the sex change process. During MT-induced sex change, serum concentrations of E(2) remained constant while T and 11-KT levels were significantly increased. Taken together, our results suggest that GtH/GtHR systems are involved in MT-induced sex change, and two signaling pathways may have distinct roles in modulating the variations of the corresponding steroid hormones in the orange-spotted grouper. Mol. Reprod. Dev. 78: 376-390, 2011. (C) 2011 Wiley-Liss, Inc.

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