Cut-dip-budding delivery system enables genetic modifications in plants without tissue culture

文献类型： 外文期刊

第一作者： Cao, Xuesong

作者： Cao, Xuesong;Song, Minglei;Huang, Boyu;Wang, Mugui;Xie, Hongtao;Ma, Ping;Li, Guofu;Cao, Xuesong;Song, Minglei;Huang, Boyu;Lang, Zhaobo;Zhu, Jian-Kang;Lang, Zhaobo;Zhu, Jian-Kang;Tian, Yifu;Peng, Jun;Zhu, Jian-Kang;Tian, Yifu;Chen, Fan;Peng, Jun;Zhu, Jian-Kang

作者机构：

期刊名称：INNOVATION （ 影响因子：32.1； 五年影响因子：32.1 ）

ISSN： 2666-6758

年卷期： 2023 年 4 卷 1 期

页码：

收录情况： SCI

摘要： Of the more than 370 000 species of higher plants in nature, fewer than 0.1% can be genetically modified due to limitations of the current gene delivery sys-tems. Even for those that can be genetically modified, the modification in-volves a tedious and costly tissue culture process. Here, we describe an extremely simple cut-dip-budding (CDB) delivery system, which uses Agro-bacterium rhizogene to inoculate explants, generating transformed roots that produce transformed buds due to root suckering. We have successfully used CDB to achieve the heritable transformation of plant species in multiple plant families, including two herbaceous plants (Taraxacum kok-saghyz and Coro-nilla varia), a tuberous root plant (sweet potato), and three woody plant spe-cies (Ailanthus altissima, Aralia elata, and Clerodendrum chinense). These plants have previously been difficult or impossible to transform, but the CDB method enabled efficient transformation or gene editing in them using a very simple explant dipping protocol, under non-sterile conditions and without the need for tissue culture. Our work suggests that large numbers of plants could be amenable to genetic modifications using the CDB method.

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