siRNA self-assembled carboxymethyl chitosan and pymetrozine nucleic pesticides for enhanced control of Sogatella furcifera: Bidirectional transduction and detoxification inhibition

文献类型： 外文期刊

第一作者： Gong, Changwei

作者： Gong, Changwei;Wang, Xuegui;Gong, Changwei;Ma, Yanxin;Peng, Anchun;Pu, Jian;Yang, Jizhi;Wang, Xuegui;Wang, Wei;Yuan, Xiangyang;Zhan, Xiaoxu

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关键词： Sogatella furcifera; Insecticide resistance management; RNAi pesticides; Insecticides delivery; Carboxymethyl chitosan

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：12.5； 五年影响因子：11.9 ）

ISSN： 0144-8617

年卷期： 2025 年 354 卷

页码：

收录情况： SCI

摘要： Sogatella furcifera (Horvath) is a significant migratory pest in southeast Asia and northern Australia, exhibiting a notable resistance to the conventional insecticides, and RNAi pesticide is a promising approach for managing resistance. However, the poor stability and difficulty in delivery hinders its wide application. Here, we present a self-assembled siRNAs, carboxymethyl chitosan (CMCS) and pymetrozine complex (abbreviated as PM-CMCS/siRNAs micro-complex) to facilitate the control of S. furcifera in rice. When the transcription factors USP and Kr-H1 were interfered with dsUSP or dsKr-H1, a mortality of 84.4 % similar to 88.9 % was observed at a concentration of 150 mu g/mL of pymetrozine, indicating a potential strategy for managing pymetrozine resistance. With the envelope of CMCS, the micro-complex effectively enhances the siRNA stability >96 h, downregulates the expression of USP, Kr-H1, and CYP6FJ3, leading to a mortality of 100 % at 100 mu g/mL pymetrozine. Moreover, the time-dependent disintegration of micro-complex induces the release of lesser PM-CMCS/siRNAs, allowing that more fluorescently labeled complexes were enriched in the phloem of rice via the apoplastic pathway. The integration of PM-CMCS/siRNAs enable precise enrichment in phloem and effective management of pest resistance by silencing their detoxification genes, and promises an environmental-friendly strategy for controlling devastating pest.

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