A direct competitive nanozyme-linked immunosorbent assay based on MnO2 nanosheets as a catalytic label for the determination of fumonisin B-1

文献类型： 外文期刊

第一作者： Cai, Xinfa

作者： Cai, Xinfa;Liang, Meijuan;Ma, Fei;Yu, Li;Li, Peiwu;Cai, Xinfa;Liang, Meijuan;Ma, Fei;Yu, Li;Li, Peiwu;Cai, Xinfa;Liang, Meijuan;Ma, Fei;Yu, Li;Li, Peiwu;Yu, Li;Li, Peiwu;Yu, Li;Li, Peiwu;Mohamed, Sherif Ramzy;Goda, Amira Abdel;Dawood, Dawood H.

作者机构：

期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）

ISSN： 1759-9660

年卷期： 2021 年 13 卷 46 期

页码：

收录情况： SCI

摘要： A direct competitive nanozyme-linked immunosorbent assay (dcNLISA) based on MnO2 nanosheets (MnO2 NSs) as a nanozyme label was developed for the highly sensitive determination of fumonisin B-1 (FB1). MnO2 NS-labeled fumonisin B-1-bovine serum albumin was easily synthesized as a competing antigen for the dcNLISA. And color changes derived from the MnO2-3,3 ',5,5 '-tetramethylbenzidine (TMB) system were exploited as the output signals of the dcNLISA. Several experimental parameters including the concentrations of the coating antibody, pH values, ionic strength and methanol concentration were optimized. Under the optimal conditions, the proposed method demonstrated a linear range (1.17-20.74 ng mL(-1)) with a reliable correlation coefficient (R-2 = 0.9989), a satisfactory limit of detection (0.63 ng mL(-1)) and high selectivity for the detection of FB1. The recoveries of FB1 in spiked corn and wheat samples were in the range of 85.31-108.16% with coefficients of variation (CVs) ranging from 6.14% to 9.23%. Meanwhile, the testing results showed good consistency (R-2 = 0.9892) between the developed dcNLISA and the reference method, liquid chromatography/mass spectrometry/mass spectrometry (LC-MS/MS) method. The proposed method was proven to be simple, sensitive, cost-effective and reliable for the screening of FB1 in cereals.

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