Genome-wide transcriptome analysis of microspore abortion initiation in radish (Raphanus sativus L.)

文献类型： 外文期刊

第一作者： Liu, Chen

作者： Liu, Chen;Fu, Weimin;Xu, Wenling;Liu, Xianxian;Wang, Shufen

作者机构：

关键词： Cytoplasmic male sterile; Microspore abortion initiation; Radish; Transcriptome

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2021 年 794 卷

页码：

收录情况： SCI

摘要： The use of male sterile lines is one of the ideal means in hybrid seed production. Despite the widespread application of Ogura cytoplasmic male sterile (CMS) lines, the molecular mechanisms remain largely unknown. In this study, histological analyses of floral buds from a CMS line 40MA and its corresponding maintainer line 40MB were conducted, which indicate that microspore abortion was initiated shortly after the tetrad stage. RNA sequencing was performed to analyze the transcriptomes of floral buds from the tetrad stage and the early microspore stages of these two lines. More than 39 million clean reads were generated for each library, and the portions mapped to the reference genome were all above 70.60%. To further analyze the differentially expressed genes (DEGs), the samples were grouped into four pairs, of which the pair of 40MA and 40MB at the early microspore stage showed the most DEGs (5100 members). According to the abnormal appearance of the tapetum cells in 40MA, a series of tapetum development related genes were screened and analyzed. In addition, a total of 623 genes with differential expressions in the tetrad stage, but not in the early microspore stage between the two lines were filtered as the microspore abortion initiation related candidates. Twelve genes were selected to validate the sequencing result by quantitative RT-PCR. In this study, we identified a number of candidate genes involved in the initiation of microspore degeneration, which may provide a new perspective to unravel the molecular mechanism of Ogura CMS.

分类号：

  • 相关文献

[1]Genome-wide transcriptome analysis of microspore abortion initiation in radish (Raphanus sativus L.). Liu, Chen,Fu, Weimin,Xu, Wenling,Liu, Xianxian,Wang, Shufen. 2021

作者其他论文 更多>>

微信小程序