农科机构知识库联盟

Molecular Characterization and Expression Analysis of a Gene Encoding 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGR) from Bipolaris eleusines, an Ophiobolin A-Producing Fungus

文献类型：外文期刊

第一作者： Zhang, Jianping

作者： Zhang, Jianping;Tang, Wei;Yang, Yongjie;Yu, Xiaoyue;Lu, Yongliang;Yu, Liuqing;Yang, Ke

作者机构：

关键词： cloning; RACE technology; transcriptome sequencing; methyl jasmonate (MJ); phytotoxin

期刊名称：JOURNAL OF FUNGI （影响因子：4.2；五年影响因子：4.5 ）

ISSN：

年卷期： 2024 年 10 卷 7 期

页码：

收录情况： SCI

摘要： Ophibolin A, a fungal sesterterpene, exerts a pivotal influence in a diverse array of biological processes, encompassing herbicidal, bactericidal, fungicidal, and cytotoxic activities. Sixty genes associated with sesterterpene compound biosynthesis were obtained from Bipolaris eleusines via transcriptome sequencing, and those closely linked to ophiobolin A biosynthesis were subsequently filtered. A gene encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) that catalyzes the first committed step of ophiobolin biosynthesis in the mevalonic acid (MVA) pathway was isolated and characterized using RACE (Rapid Amplification of cDNA Ends) technology from ophiobolin A-producing fungus, B. eleusines. The full-length cDNA of the B. eleusines HMGR gene (BeHMGR) was 3906 bp and contained a 3474 bp open reading frame (ORF) encoding 1157 amino acids. Sequence analysis revealed that deduced BeHMGR had high homology to the known HMGRs from Pyrenophora tritici-repentis and Leptosphaeria maculans. It had a calculated molecular mass of about 124.65 kDa and an isoelectric point (pI) of 6.90. It contained two putative HMG-CoA-binding motifs and two NADP(H)-binding motifs. Induced expression analysis of the BeHMGR gene by methyl jasmonate treatment using quantitative fluorescence PCR showed that it significantly elevated after 3 h of methyl jasmonate treatment, peaked at 6 h, and then gradually decreased. This demonstrates that BeHMGR gene expression is induced by methyl jasmonate.

分类号：

相关文献

作者其他论文更多>>

Vis-NIR and NIR hyperspectral imaging combined with convolutional neural network with attention module for flaxseed varieties identification

作者：Zhu, Dongyu;Han, Junying;Liu, Chengzhong;Zhang, Jianping;Qi, Yanni

关键词：Hyperspectral imaging; Flaxseed; Identification; Convolutional neural network; Channel attention and transformer modules
Identification of ZmSNAC06, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in Arabidopsis

作者：Wang, Fei;Yang, Ruisi;Luo, Ping;Wang, Houwen;Zhang, Runze;Li, Wenzhe;Yang, Ke;Xu, Xinlong;Hao, Zhuanfang;Li, Xinhai;Chen, Yong

关键词：maize (Zea mays L.); ZmSNAC06; transcription factor; drought tolerance
Protein Phosphatases MoPtc5, MoPtc1, and MoPtc2 Contribute to the Vegetative Growth, Stress Adaptation, and Virulence of Magnaporthe oryzae

作者：Biregeya, Jules;Otieno, Frankline Jagero;Mabeche, Anjago Wilfred;Felix, Abah;Aimable, Nsanzinshuti;Abubakar, Yakubu Saddeeq;Aron, Osakina;Lu, Guodong;Wang, Zonghua;Tang, Wei;Biregeya, Jules;Hong, Yonghe;Chen, Meilian;Wang, Zonghua

关键词：Magnaporthe oryzae; synergistic; stress tolerance; protein phosphatases; pathogenesis
Genome-Wide Identification and Expression Profiling of Dehydration-Responsive Element-Binding Family Genes in Flax (Linum usitatissimum L.)

作者：Wang, Yan;Niu, Yamin;Lu, Nan;Hu, Zuyu;Liu, Zigang;Zhang, Jianping;Wang, Yan;Qi, Yanni;Wang, Limin;Xu, Chenmeng;Li, Wenjuan;Dang, Zhao;Zhao, Wei;Wang, Ping;Xie, Yaping;Niu, Yamin;Lu, Nan;Hu, Zuyu;Zhang, Jianping;Liu, Zigang

关键词：flax; DREB; transcription factors; abiotic stress; expression analysis
Genetic analysis and identification of major locus and candidate genes linked to thousand-seed weight in flax (Linum usitatissimum)

作者：Qi, Yanni;Wang, Limin;Li, Wenjuan;Dang, Zhao;Zhao, Wei;Wang, Ping;Xie, Yaping;Xu, Chenmeng;Wang, Yan;Zhang, Jianping;Wang, Wei

关键词：Flax; TSW; BSA-seq; Fine mapping; qRT-PCR
Transcriptomics and EtSRG1 Functional Analyses Provide New Insights Into the Regulatory Mechanisms of Secondary Appressoria Formation of Exserohilum turcicum

作者：Fang, Ya-Li;Li, Jing;Yang, Xin-Yan;Chen, Yi-Zhe;Ding, Zhi-Chao;Zhou, Jian-Bo;Zhang, Zhi-Jia;Wang, De-Long;Fang, Ya-Li;Tang, Wei

关键词：appressorium; Exserohilum turcicum; NmrA-like domain; northern corn leaf blight; transcriptomics
Characterization of Flexusin A, a Novel Circular Bacteriocin Produced by Marine Bacterium Bacillus flexus R29-2

作者：Qiao, Xiaoni;Sun, Xiaowen;Wang, Shuting;Tang, Wei;Tang, Tao;Zhang, Jun;Qiao, Xiaoni;Sun, Xiaowen;Wang, Shuting;Tang, Wei;Tang, Tao;He, Zengguo;Zhai, Chen;Tang, Wei;Tang, Tao;He, Zengguo

关键词：Bacillus flexus R29-2; circular bacteriocin; characterization; leader peptide; mechanism of action

Molecular Characterization and Expression Analysis of a Gene Encoding 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGR) from Bipolaris eleusines, an Ophiobolin A-Producing Fungus

作者其他论文 更多>>

Vis-NIR and NIR hyperspectral imaging combined with convolutional neural network with attention module for flaxseed varieties identification

Identification of ZmSNAC06, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in Arabidopsis

Protein Phosphatases MoPtc5, MoPtc1, and MoPtc2 Contribute to the Vegetative Growth, Stress Adaptation, and Virulence of Magnaporthe oryzae

Genome-Wide Identification and Expression Profiling of Dehydration-Responsive Element-Binding Family Genes in Flax (Linum usitatissimum L.)

Genetic analysis and identification of major locus and candidate genes linked to thousand-seed weight in flax (Linum usitatissimum)

Transcriptomics and EtSRG1 Functional Analyses Provide New Insights Into the Regulatory Mechanisms of Secondary Appressoria Formation of Exserohilum turcicum

Characterization of Flexusin A, a Novel Circular Bacteriocin Produced by Marine Bacterium Bacillus flexus R29-2

作者其他论文更多>>