The role of caspase 3 in the mud crab (Scylla paramamosain) after Vibrio parahaemolyticus infection

文献类型： 外文期刊

第一作者： Cheng, Chang-Hong

作者： Cheng, Chang-Hong;Ma, Hong-Ling;Liu, Guang-Xin;Deng, Yi-Qin;Feng, Juan;Jie, Yu-Kun;Guo, Zhi-Xun;Liu, Xiu-Ze

作者机构：

关键词： Scylla paramamosain; Caspase 3; Vibrio parahaemolyticus; Apoptosis

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2021 年 118 卷

页码：

收录情况： SCI

摘要： Apoptosis plays essential roles in the immune defense mechanism against pathogen infection. Caspase 3 is a family of cysteine proteases involved in apoptosis and the immune response. In this study, the full-length of mud crab (Scylla paramamosain) caspase 3 (designated as Sp-caspase 3) was cloned and characterized. The open reading frame of Sp-caspase 3 was comprised a 1035 bp, which encoded a putative protein of 344 amino acids. Sp-caspase 3 was ubiquitously expressed in various tissues with a high-level expression in hemocytes. Cellular localization analysis revealed that Sp-caspase 3 was located in the cytoplasm and nucleus. Over-expression of Spcaspase 3 could induce cell apoptosis. In addition, V. Parahaemolyticus infection induced the relative expression of caspase-3 mRNA and increased caspase-3 activity. Knocking down Sp-caspase 3 in vivo significantly reduced cell apoptosis and increased mortality of mud crab after V. parahaemolyticus infection. These results indicated that Sp-caspase 3 played important roles in the immune response and apoptosis against bacterial infection.

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