DNA methylation remodeled amino acids biosynthesis regulates flower senescence in carnation (Dianthus caryophyllus)

文献类型： 外文期刊

第一作者： Feng, Shan

作者： Feng, Shan;Huang, Zhiheng;Wang, Ruiming;Yuan, Xinyi;Sun, Zheng;Tan, Hualiang;Zhong, Linlin;Cheng, Yunjiang;Bao, Manzhu;Zhang, Fan;Feng, Shan;Huang, Zhiheng;Wang, Ruiming;Yuan, Xinyi;Sun, Zheng;Tan, Hualiang;Cheng, Yunjiang;Zhang, Fan;Feng, Shan;Huang, Zhiheng;Wang, Ruiming;Yuan, Xinyi;Sun, Zheng;Tan, Hualiang;Zhong, Linlin;Cheng, Yunjiang;Zhang, Fan;Jiang, Xinyu;Song, Qingxin;Li, Fan;Li, Shenchong;Wang, Jihua;Zhang, Fan;Li, Fan;Li, Shenchong;Wang, Jihua;Bao, Manzhu;Zhang, Fan;Bao, Manzhu;Zhang, Fan;Qiao, Hong;Qiao, Hong

作者机构：

关键词： amino acid; carnation; Dianthus caryophyllus L.; DNA methylation; epigenetic regulation; ethylene; flower senescence; ROS1

期刊名称：NEW PHYTOLOGIST （ 影响因子：9.4； 五年影响因子：10.5 ）

ISSN： 0028-646X

年卷期： 2024 年 241 卷 4 期

页码：

收录情况： SCI

摘要： center dot Dynamic DNA methylation regulatory networks are involved in many biological processes. However, how DNA methylation patterns change during flower senescence and their relevance with gene expression and related molecular mechanism remain largely unknown.center dot Here, we used whole genome bisulfite sequencing to reveal a significant increase of DNA methylation in the promoter region of genes during natural and ethylene-induced flower senescence in carnation (Dianthus caryophyllus L.), which was correlated with decreased expression of DNA demethylase gene DcROS1. Silencing of DcROS1 accelerated while overexpression of DcROS1 delayed carnation flower senescence.center dot Moreover, among the hypermethylated differentially expressed genes during flower senescence, we identified two amino acid biosynthesis genes, DcCARA and DcDHAD, with increased DNA methylation and reduced expression in DcROS1 silenced petals, and decreased DNA methylation and increased expression in DcROS1 overexpression petals, accompanied by decreased or increased amino acids content. Silencing of DcCARA and DcDHAD accelerates carnation flower senescence. We further showed that adding corresponding amino acids could largely rescue the senescence phenotype of DcROS1, DcCARA and DcDHAD silenced plants.center dot Our study not only demonstrates an essential role of DcROS1-mediated remodeling of DNA methylation in flower senescence but also unravels a novel epigenetic regulatory mechanism underlying DNA methylation and amino acid biosynthesis during flower senescence.

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