Optimization of infection parameters improves Bombyx mori nucleopolyhedrovirus invasion efficiency

文献类型： 外文期刊

第一作者： Xu, Ying

作者： Xu, Ying;Liu, Na;Yang, Fan;Wang, Xueya;Huang, Jinshan;Shen, Xingjia;Hao, Bifang;Huang, Jinshan;Shen, Xingjia;Hao, Bifang

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关键词： Baculovirus; MOI; Incubation time; Infection efficiency; Protein expression

期刊名称：BIOLOGIA （ 影响因子：1.35； 五年影响因子：1.14 ）

ISSN： 0006-3088

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收录情况： SCI

摘要： Although the baculovirus-mediated foreign protein expression system has been extensively documented, system investigation of the optimal infection conditions remains elusive. In this study, a recombinant baculovirus vBmBacJS13-egfp was applied for systematic infection of Bombyx mori nucleopolyhedrovirus (BmNPV). We revealed that incubation time and multiplicity of infection (MOI) were the crucial factors affecting the efficiency of virus infection and protein expression. Given the appropriate MOI, BmNPV infection efficiency was increased with elongation of incubation time; the optimal incubation time for BmNPV entry into host cells was about 4 h. Alternatively, an increasing MOI could shorten the incubation time of virus invasion to achieve similar infection efficiency and heterogenous proteins expression levels. This study laid a foundation for the study of the functional genome of BmNPV and provided important parameters for significant improvement on the expression level of heterogenous proteins using the BmNPV-cell expression system.

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